DTNBP1 antibody (AA 1-351)

Catalog No. ABIN7869237

The Rabbit Polyclonal anti-DTNBP1 antibody is suitable to detect DTNBP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for DTNBP1 antibody (AA 1-351) (ABIN7869237)

Target: DTNBP1 (Dystrobrevin Binding Protein 1 (DTNBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DTNBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-DTNBP1 Antibody

Binding Specificity: AA 1-351

Purpose: DTNBP1 Antibody / Dysbindin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Dysbindin/DTNBP1 recombinant protein (Position: M1-S351) was used as the immunogen for the DTNBP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DTNBP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DTNBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DTNBP1

Target: DTNBP1 (Dystrobrevin Binding Protein 1 (DTNBP1))

Alternative Name: DTNBP1

Background: DTNBP1 antibody detects Dystrobrevin-binding protein 1 (commonly known as Dysbindin), a cytoplasmic protein involved in vesicle trafficking, synaptic function, and organelle biogenesis. Encoded by the DTNBP1 gene on chromosome 6p22.3, this protein is a core component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). Through its scaffolding and trafficking functions, Dysbindin regulates endosomal transport, melanosome formation, and neurotransmitter release, particularly within neuronal and pigment cells.

Dysbindin interacts with other BLOC-1 subunits, including SNAPIN, MUTED, and BLOC1S proteins, coordinating actin-based vesicular transport. In neurons, it contributes to synaptic vesicle recycling and glutamatergic signaling, influencing synaptic plasticity and cognitive processes. Dysbindin localizes to endosomes and synaptic vesicles, reflecting its role in membrane trafficking and receptor recycling.

The DTNBP1 antibody is widely used in neuroscience, cell biology, and genetic disease research to examine endosomal transport, synaptic vesicle dynamics, and intracellular trafficking. Western blot analysis identifies a 40 kilodalton band corresponding to Dysbindin, while immunofluorescence shows punctate cytoplasmic staining consistent with endosomal localization. This antibody supports investigations into molecular mechanisms of vesicle regulation and neurotransmission.

Loss-of-function mutations or reduced expression of DTNBP1 have been associated with Hermansky-Pudlak syndrome type 7 and susceptibility to schizophrenia, linking Dysbindin to synaptic homeostasis and neurodevelopment. Dysbindin also influences muscle membrane stability by binding to dystrobrevins. The DTNBP1 antibody provides a dependable reagent for exploring vesicle trafficking, neurological function, and genetic disorders. NSJ Bioreagents validates this antibody for its applications, ensuring reproducible detection in cellular and neurological research.

UniProt: Q96EV8

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling