CREB3 antibody (AA 1-352)

Catalog No. ABIN7869239

This Rabbit Polyclonal antibody specifically detects CREB3 in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CREB3 antibody (AA 1-352) (ABIN7869239)

Target: CREB3 (CAMP Responsive Element Binding Protein 3 (CREB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CREB3 Antibody

Binding Specificity: AA 1-352

Purpose: CREB3 Antibody / Cyclic AMP-responsive element-binding protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CREB3 recombinant protein (Position: M1-K352) was used as the immunogen for the CREB3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CREB3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CREB3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CREB3

Target: CREB3 (CAMP Responsive Element Binding Protein 3 (CREB3))

Alternative Name: CREB3

Background: CREB3 antibody detects Cyclic AMP-responsive element-binding protein 3, a transcription factor involved in the unfolded protein response and endoplasmic reticulum (ER) stress regulation. The UniProt recommended name is Cyclic AMP-responsive element-binding protein 3 (CREB3). This ER membrane-bound transcription factor controls gene expression related to protein folding, trafficking, and secretion.

Functionally, CREB3 antibody identifies a 371-amino-acid protein anchored in the ER membrane with a cytoplasmic basic leucine zipper (bZIP) domain. Upon ER stress, the N-terminal transcriptional domain of CREB3 is cleaved and translocated to the nucleus, where it activates target genes involved in secretion and Golgi maintenance. CREB3 also regulates acute phase response genes and collagen biosynthesis.

The CREB3 gene is located on chromosome 9p13.3 and is highly expressed in secretory tissues such as liver, pancreas, and intestine. It plays a central role in adapting to increased secretory load and maintaining ER homeostasis under stress conditions.

Pathologically, dysregulation of CREB3 contributes to metabolic disorders, neurodegeneration, and cancer progression by disturbing ER stress response pathways. Overexpression can enhance tumor invasion by modulating extracellular matrix and stress-related signaling genes. Research using CREB3 antibody supports studies in transcriptional regulation, ER stress, and secretory pathway biology.

CREB3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors and stress response regulators. NSJ Bioreagents provides CREB3 antibody reagents optimized for research in cellular homeostasis, transcription, and disease adaptation.

Structurally, Cyclic AMP-responsive element-binding protein 3 contains a bZIP domain for DNA binding and dimerization, along with a luminal stress-sensing region. This antibody enables investigation of CREB3's role in ER stress signaling and transcriptional control.

UniProt: O43889

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Maintenance of Protein Location, Unfolded Protein Response