FOXI1 antibody (AA 1-378)

Catalog No. ABIN7869272

This Rabbit Polyclonal antibody specifically detects FOXI1 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for FOXI1 antibody (AA 1-378) (ABIN7869272)

Target: FOXI1 (Forkhead Box I1 (FOXI1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FOXI1 Antibody

Binding Specificity: AA 1-378

Purpose: FOXI1 Antibody / Forkhead box protein I1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human FOXI1 recombinant protein (Position: M1-V378) was used as the immunogen for the FOXI1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FOXI1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FOXI1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FOXI1

Target: FOXI1 (Forkhead Box I1 (FOXI1))

Alternative Name: FOXI1

Background: FOXI1 antibody targets Forkhead box protein I1, a member of the FOX family of transcription factors characterized by a conserved forkhead or 'winged helix' DNA-binding domain. The FOXI1 gene, located on chromosome 5q35.1, encodes a nuclear transcription factor that regulates ion transport and epithelial differentiation, particularly in the inner ear, kidney, and epididymis. FOXI1 controls expression of genes involved in acid-base balance and fluid homeostasis, including the vacuolar H+-ATPase subunit and pendrin (SLC26A4), a chloride/bicarbonate exchanger essential for maintaining luminal pH .

Structurally, FOXI1 contains a 110-amino acid forkhead domain that interacts with specific DNA motifs to modulate transcriptional activity. It functions as a transcriptional activator for genes expressed in intercalated cells of the distal nephron and cochlear epithelial cells. In the inner ear, FOXI1 contributes to endolymphatic ion transport and hearing development. In the kidney, loss of FOXI1 disrupts acid secretion, resulting in distal renal tubular acidosis (dRTA). Genetic mutations in FOXI1 have been linked to autosomal recessive dRTA and sensorineural hearing loss, demonstrating its dual role in renal and auditory physiology.

FOXI1 also participates in epithelial polarity regulation and may influence Wnt and Notch signaling pathways during organogenesis. Dysregulation of FOXI1 expression has been reported in certain cancers, where it may contribute to altered epithelial transport and cell differentiation. Functional studies suggest FOXI1 acts as a transcriptional regulator for ion transport proteins and can modulate developmental genes associated with morphogenesis of the ear and kidney.

Immunohistochemical staining using FOXI1 antibody reveals nuclear localization in renal intercalated cells, cochlear epithelium, and reproductive tissues. FOXI1 antibody from NSJ Bioreagents provides an essential reagent for research in transcriptional regulation, epithelial physiology, and hereditary renal and auditory disorders.

UniProt: Q12951