LYAR antibody (AA 1-379)

Catalog No. ABIN7869273

The Rabbit Polyclonal anti-LYAR antibody is suitable to detect LYAR in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IP, ICC and IF.

Quick Overview for LYAR antibody (AA 1-379) (ABIN7869273)

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-LYAR Antibody

Binding Specificity: AA 1-379

Purpose: LYAR Antibody / Cell growth-regulating nucleolar protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human LYAR recombinant protein (Position: M1-K379) was used as the immunogen for the LYAR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the LYAR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the LYAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LYAR

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Alternative Name: LYAR

Target Type: Antibody

Background: The LYAR antibody targets Cell growth-regulating nucleolar protein, a zinc finger nucleolar protein encoded by the LYAR gene. Originally identified in human leukemia cells, LYAR plays roles in ribosome biogenesis, rRNA processing, and cell proliferation. Cell growth-regulating nucleolar protein associates with pre-ribosomal complexes and contributes to ribosomal subunit maturation and export. The LYAR antibody provides an essential tool for investigating nucleolar function, ribosome assembly, and growth regulation in both normal and transformed cells.

Cell growth-regulating nucleolar protein contains a C2HC-type zinc finger motif and a nuclear localization signal, directing it to the nucleolus where ribosome synthesis occurs. It participates in the early stages of 60S ribosomal subunit formation by binding pre-rRNA and ribonucleoprotein complexes. The LYAR antibody enables detection of this nucleolar marker, supporting studies into the molecular events that control ribosome production and cellular growth rates. Its expression correlates with proliferative activity in rapidly dividing cells.

LYAR has emerged as a multifunctional protein involved in transcriptional regulation and cell cycle progression. It interacts with nucleolin and other nucleolar proteins to coordinate ribosomal RNA processing. Beyond ribosome synthesis, Cell growth-regulating nucleolar protein influences p53 signaling and apoptosis by modulating nucleolar stress responses. The LYAR antibody supports analysis of these pathways, helping define how nucleolar integrity links to cell cycle checkpoints and stress-induced growth arrest.

Overexpression of LYAR has been observed in several cancers, including colorectal, prostate, and hepatocellular carcinoma. It promotes cell proliferation and survival by enhancing rRNA synthesis and suppressing apoptosis. The LYAR antibody enables quantitative assessment of its expression in tumor tissues and cell models, aiding investigation into oncogenic mechanisms involving ribosome biogenesis. In contrast, depletion of LYAR impairs ribosomal maturation and reduces cell growth, underscoring its importance for proliferation control.

The LYAR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing distinct nucleolar staining consistent with its localization. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for use in cell biology, cancer research, and molecular genetics. By supporting precise detection of Cell growth-regulating nucleolar protein, the LYAR antibody advances understanding of ribosome biogenesis, nucleolar stress, and their contributions to tumorigenesis.

UniProt: Q9NX58