NSUN4 antibody (AA 1-379)

Catalog No. ABIN7869274

The Rabbit Polyclonal anti-NSUN4 antibody (ABIN7869274) specifically detects NSUN4 in ELISA and WB. The antibody is reactive with Human samples.

Quick Overview for NSUN4 antibody (AA 1-379) (ABIN7869274)

Target: NSUN4 (NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-NSUN4 Antibody

Binding Specificity: AA 1-379

Purpose: NSUN4 Antibody / NOP2/Sun RNA methyltransferase 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NSUN4 recombinant protein (Position: M1-K379) was used as the immunogen for the NSUN4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NSUN4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NSUN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NSUN4

Target: NSUN4 (NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4))

Alternative Name: NSUN4

Background: NSUN4 antibody detects NOP2/Sun RNA methyltransferase 4, encoded by the NSUN4 gene. NOP2/Sun RNA methyltransferase 4 is a mitochondrial rRNA methyltransferase that modifies 12S rRNA and plays a critical role in ribosome assembly and mitochondrial translation. NSUN4 antibody provides researchers with a specific reagent for studying RNA modification, mitochondrial function, and disease associated with mitochondrial dysfunction.

NOP2/Sun RNA methyltransferase 4 belongs to the NSUN family of RNA cytosine methyltransferases. Research using NSUN4 antibody has shown that it catalyzes 5-methylcytosine modification of mitochondrial 12S rRNA. This modification is required for proper folding of ribosomal RNA and stability of the small ribosomal subunit. NSUN4 also interacts with MTERF4, forming a complex that regulates assembly of the mitochondrial large ribosomal subunit.

Studies with NSUN4 antibody have revealed that knockout of NSUN4 leads to defective mitochondrial ribosome biogenesis, impaired oxidative phosphorylation, and embryonic lethality in model organisms. These findings emphasize its essential role in mitochondrial translation and energy metabolism. Without NSUN4, mitochondrial protein synthesis collapses, resulting in severe cellular dysfunction.

Dysregulation of NOP2/Sun RNA methyltransferase 4 has been linked to human disease. Research using NSUN4 antibody has shown that mutations cause mitochondrial translation defects, leading to neurodevelopmental delay and cardiomyopathy. In cancer biology, altered NSUN4 expression may contribute to metabolic reprogramming, where cancer cells adapt mitochondrial translation to support growth. These observations highlight the dual importance of NSUN4 in normal physiology and pathology.

NSUN4 antibody is widely used in immunofluorescence, western blotting, and immunohistochemistry. Immunofluorescence demonstrates mitochondrial localization, western blotting quantifies protein levels in mitochondrial fractions, and immunohistochemistry shows expression in tissues with high energy demand such as heart and brain. These applications make NSUN4 antibody indispensable in mitochondrial biology research.

By supplying validated NSUN4 antibody reagents, NSJ Bioreagents supports studies into RNA modification, mitochondrial translation, and disease. Detection of NOP2/Sun RNA methyltransferase 4 provides researchers with insight into how RNA modifying enzymes regulate mitochondrial function and pathology.

UniProt: Q96CB9