NLE1 antibody (AA 1-385)

Catalog No. ABIN7869280

The Rabbit Polyclonal anti-NLE1 antibody (ABIN7869280) specifically detects NLE1 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NLE1 antibody (AA 1-385) (ABIN7869280)

Target: NLE1 (Notchless Homolog 1 (NLE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NLE1 Antibody

Binding Specificity: AA 1-385

Purpose: NLE1 Antibody / Notchless protein homolog 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NLE1 recombinant protein (Position: M1-R385) was used as the immunogen for the NLE1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NLE1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NLE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NLE1

Target: NLE1 (Notchless Homolog 1 (NLE1))

Alternative Name: NLE1

Background: The NLE1 antibody targets Notchless protein homolog 1, a nucleolar assembly factor encoded by the NLE1 gene. Notchless protein homolog 1 is required for ribosome biogenesis, pre-60S subunit maturation, and proper cell-cycle progression. Originally identified for its genetic interaction with the Notch pathway, NLE1 participates in both ribosomal RNA processing and developmental signaling. The NLE1 antibody enables researchers to study nucleolar organization, protein-synthesis control, and cross-talk between growth and differentiation pathways.

Notchless protein homolog 1 contains WD-repeat motifs that facilitate interactions with nucleolar proteins involved in ribosome assembly, including NOP7 and RPF2. It promotes 60S subunit maturation by assisting in rRNA folding and ribosomal protein incorporation. The NLE1 antibody supports localization studies showing strong nucleolar enrichment, consistent with its essential role in ribosome production. Depletion of NLE1 results in defective ribosome biogenesis, p53 activation, and cell-cycle arrest.

During embryonic development, Notchless protein homolog 1 influences cell-fate determination through modulation of Notch receptor signaling. Loss of NLE1 disrupts somite segmentation and neurogenesis in animal models. The NLE1 antibody allows analysis of these regulatory interactions, helping delineate how nucleolar activity intersects with developmental gene networks. Its dual role in ribosome formation and signaling regulation underscores the integration of biosynthetic and morphogenetic processes.

In cancer research, altered ribosome biogenesis and nucleolar stress contribute to tumorigenesis. Notchless protein homolog 1 expression correlates with proliferative capacity, making it a useful marker for studies of nucleolar dynamics in malignant tissues. The NLE1 antibody supports evaluation of nucleolar size, rRNA synthesis rate, and ribosomal assembly integrity in cancer and stress-response models.

The NLE1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing prominent nucleolar staining. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for ribosome biology, developmental genetics, and cancer research. By enabling detailed analysis of Notchless protein homolog 1, the NLE1 antibody advances understanding of ribosomal assembly pathways and their coordination with developmental and oncogenic signaling.

UniProt: Q9NVX2