MBTD1 antibody (AA 1-420)

Catalog No. ABIN7869339

The Rabbit Polyclonal anti-MBTD1 antibody is suitable to detect MBTD1 in samples from Human. It has been validated for WB, ELISA, FACS, ICC and IF.

Quick Overview for MBTD1 antibody (AA 1-420) (ABIN7869339)

Target: MBTD1 (Mbt Domain Containing 1 (MBTD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBTD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MBTD1 Antibody

Binding Specificity: AA 1-420

Purpose: MBTD1 Antibody / Malignant Brain Tumor domain containing 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MBTD1 recombinant protein (Position: M1-D420) was used as the immunogen for the MBTD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MBTD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MBTD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MBTD1

Target: MBTD1 (Mbt Domain Containing 1 (MBTD1))

Alternative Name: MBTD1

Background: MBTD1 antibody detects Malignant Brain Tumor domain containing 1, encoded by the MBTD1 gene on chromosome 17q21.33. MBTD1 antibody is widely used in research on chromatin remodeling, transcription regulation, and DNA repair. MBTD1 belongs to the malignant brain tumor (MBT) domain-containing protein family, characterized by their ability to bind methylated histone lysine residues. MBTD1 functions in transcriptional repression and DNA damage response, integrating chromatin state with cellular processes.

Structurally, MBTD1 is a ~65 kDa protein containing four MBT domains that recognize methylated histone marks, particularly H4K20me1 and H4K20me2. It also features protein-protein interaction motifs that recruit corepressor complexes. MBTD1 is localized in the nucleus and associates with chromatin at transcriptionally repressed regions. Alternative isoforms expand regulatory diversity.

Functionally, MBTD1 regulates chromatin compaction and gene silencing. It participates in Polycomb group-mediated repression, maintaining developmental gene expression programs. MBTD1 also contributes to DNA repair, particularly homologous recombination, by recruiting repair factors to sites of double-strand breaks. Researchers employ MBTD1 antibody to study chromatin regulation, transcriptional repression, and DNA repair pathways.

Clinically, MBTD1 has been implicated in cancer and developmental disorders. Chromosomal translocations involving MBTD1 and ZMYND11 generate fusion proteins associated with acute myeloid leukemia. MBTD1 mutations are linked to neurodevelopmental defects, reflecting its essential role in gene regulation. Because of its role in histone mark recognition, MBTD1 is being investigated as a potential target in epigenetic therapies. NSJ Bioreagents offers MBTD1 antibody to support chromatin and cancer research.

Experimentally, MBTD1 antibody is used in western blotting to detect the ~65 kDa protein, in immunohistochemistry to study nuclear expression, and in chromatin immunoprecipitation to analyze binding at histone-modified loci. Co-immunoprecipitation with MBTD1 antibody identifies corepressor complexes and repair factors.

UniProt: Q05BQ5