PLIN5 antibody (AA 1-425)

Catalog No. ABIN7869347

This Rabbit Polyclonal antibody specifically detects PLIN5 in ELISA, WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PLIN5 antibody (AA 1-425) (ABIN7869347)

Target: PLIN5 (Perilipin 5 (PLIN5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLIN5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PLIN5 Antibody

Binding Specificity: AA 1-425

Purpose: PLIN5 Antibody / Perilipin 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLIN5 recombinant protein (Position: M1-D425) was used as the immunogen for the PLIN5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLIN5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLIN5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PLIN5

Target: PLIN5 (Perilipin 5 (PLIN5))

Alternative Name: PLIN5

Background: PLIN5 antibody detects Perilipin-5, encoded by the PLIN5 gene on chromosome 19p13.3. PLIN5 antibody is commonly used to study this lipid droplet-associated protein that regulates lipid storage, oxidation, and energy metabolism. PLIN5 belongs to the perilipin family, which includes PLIN1-5, and is highly expressed in oxidative tissues such as heart, skeletal muscle, brown adipose tissue, and liver. Its primary function is to coat lipid droplets, protect stored triglycerides, and coordinate lipolysis and fatty acid utilization.

Structurally, PLIN5 contains amphipathic helices that allow binding to lipid droplet surfaces. These regions protect lipid stores from uncontrolled hydrolysis by shielding them from lipases. PLIN5 also interacts with enzymes such as adipose triglyceride lipase (ATGL) and comparative gene identification-58 (CGI-58), serving as a regulatory scaffold for lipolysis. It recruits oxidative enzymes and mitochondria to lipid droplets, promoting efficient channeling of fatty acids into beta-oxidation pathways. This coordination is critical in energy-demanding tissues, where fatty acid metabolism fuels contractile and thermogenic activity.

Functionally, PLIN5 balances lipid storage and oxidation. Overexpression increases lipid droplet size but enhances mitochondrial fatty acid oxidation, preventing lipotoxicity. Knockout mice lacking PLIN5 exhibit impaired fatty acid metabolism, cardiac dysfunction, and susceptibility to metabolic stress. PLIN5 also plays a role in stress signaling by regulating lipid-derived reactive oxygen species. Its ability to integrate lipid droplet biology with mitochondrial function highlights its importance in energy homeostasis.

Clinically, dysregulation of PLIN5 is linked to metabolic diseases. Overexpression in the liver contributes to nonalcoholic fatty liver disease, while reduced expression impairs cardiac energy metabolism. PLIN5 variants are associated with altered lipid profiles, insulin resistance, and cardiovascular disease risk. Elevated PLIN5 expression has been observed in endurance-trained athletes, suggesting adaptive roles in enhancing oxidative capacity. Researchers use PLIN5 antibody to analyze its expression in metabolic tissues and disease models.

Experimentally, PLIN5 antibody is used in western blotting to detect the ~51 kDa protein, in immunofluorescence to label lipid droplets, and in immunohistochemistry to evaluate tissue distribution. Co-immunoprecipitation with PLIN5 antibody demonstrates its interactions with ATGL and CGI-58, linking it to lipolysis regulation. NSJ Bioreagents provides PLIN5 antibody as a reliable tool for studies in metabolism, cardiovascular biology, and energy regulation.

UniProt: Q00G26