APC antibody (AA 1-433)

Catalog No. ABIN7869357

The Mouse Monoclonal anti-APC antibody is suitable to detect APC in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for APC antibody (AA 1-433) (ABIN7869357)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ALi 12-28

Product Details anti-APC Antibody

Binding Specificity: AA 1-433

Purpose: Adenomatous polyposis coli Antibody / APC

Purification: Protein G affinity chromatography

Immunogen: A recombinant human partial protein (amino acids 1-433) was used as the immunogen for the Adenomatous polyposis coli antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Adenomatous polyposis coli antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Adenomatous polyposis coli antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: Adenomatous polyposis coli

Background: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization