SOX9 antibody (AA 1-496)

Catalog No. ABIN7869416

This Rabbit Polyclonal antibody specifically detects SOX9 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SOX9 antibody (AA 1-496) (ABIN7869416)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SOX9 Antibody

Binding Specificity: AA 1-496

Purpose: SOX9 Antibody / SRY-box transcription factor 9

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SOX9 recombinant protein (Position: M1-Q496) was used as the immunogen for the SOX9 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SOX9 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SOX9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: SOX9 antibody detects SRY-box transcription factor 9, a transcriptional regulator that controls chondrogenesis, sex determination, and stem cell maintenance. The UniProt recommended name is SRY-box transcription factor 9 (SOX9). This protein belongs to the SOX (SRY-related HMG-box) family of transcription factors, characterized by a high-mobility group DNA-binding domain that recognizes specific enhancer sequences.

Functionally, SOX9 antibody identifies a 509-amino-acid nuclear protein that binds to promoter and enhancer regions of cartilage-specific genes such as COL2A1 and ACAN. SOX9 forms dimers through its HMG domain and recruits transcriptional coactivators to drive cartilage matrix production and skeletal formation. It also plays roles in sex differentiation by activating AMH (anti-Mi 1/2llerian hormone) transcription during gonadal development.

The SOX9 gene is located on chromosome 17q24.3 and is expressed in developing cartilage, gonads, and neural crest-derived tissues. SOX9 is a master regulator of chondrocyte differentiation and is essential for maintaining progenitor cell identity in multiple lineages.

Pathologically, mutations or haploinsufficiency of SOX9 cause campomelic dysplasia, a severe skeletal malformation syndrome with sex reversal. Aberrant SOX9 expression also contributes to cancer progression and tissue fibrosis. Research using SOX9 antibody supports studies in developmental biology, transcription regulation, and regenerative medicine.

SOX9 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors involved in chondrogenesis and lineage specification. NSJ Bioreagents provides SOX9 antibody reagents optimized for studies in cartilage biology, stem cell differentiation, and developmental genetics.

Structurally, SRY-box transcription factor 9 contains an HMG DNA-binding domain and transactivation regions that mediate chromatin remodeling and transcriptional activation. This antibody supports detailed analysis of SOX9's regulatory mechanisms in development and disease.

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development