RORC antibody (AA 1-50)

Catalog No. ABIN7869425

This Mouse Monoclonal antibody specifically detects RORC in IHC (p). It exhibits reactivity toward Human.

Quick Overview for RORC antibody (AA 1-50) (ABIN7869425)

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RORC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RORC-2941

Product Details anti-RORC Antibody

Binding Specificity: AA 1-50

Purpose: RORC Antibody / ROR gamma

Purification: Protein G affinity chromatography

Immunogen: Recombinant human RORC protein was used as the immunogen for the RORC antibody. The epitope has been found to be with amino acids 1-50.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the RORC antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the RORC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Alternative Name: RORC

Background: This MAb recognizes a protein of 63 kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR alpha and ROR gamma are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50 % amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered 'orphan receptors,' due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

UniProt: P51449

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway