Sorting Nexin 2 antibody (AA 1-506)

Catalog No. ABIN7869441

The Rabbit Polyclonal anti-Sorting Nexin 2 antibody is suitable to detect Sorting Nexin 2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for Sorting Nexin 2 antibody (AA 1-506) (ABIN7869441)

Target: Sorting Nexin 2 (SNX2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sorting Nexin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Sorting Nexin 2 Antibody

Binding Specificity: AA 1-506

Purpose: SNX2 Antibody / Sorting nexin 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SNX2 recombinant protein (Position: M1-K506) was used as the immunogen for the SNX2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SNX2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SNX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Sorting Nexin 2

Target: Sorting Nexin 2 (SNX2)

Alternative Name: SNX2

Background: SNX2 antibody detects Sorting nexin-2, a membrane-associated protein involved in endosomal sorting, receptor trafficking, and retrograde transport from endosomes to the trans-Golgi network. The UniProt recommended name is Sorting nexin-2 (SNX2). This protein belongs to the sorting nexin family characterized by a phosphoinositide-binding PX (phox homology) domain that mediates association with endosomal membranes rich in phosphatidylinositol 3-phosphate.

Functionally, SNX2 antibody identifies a 521-amino-acid cytoplasmic protein that localizes to early and recycling endosomes. SNX2 functions as a key component of the retromer complex, which retrieves cargo proteins from endosomes for recycling to the Golgi apparatus or plasma membrane. It works in concert with other retromer subunits such as VPS26, VPS29, and VPS35 to ensure proper sorting and trafficking of membrane receptors including mannose-6-phosphate receptor and Wntless.

The SNX2 gene is located on chromosome 5q23.3 and encodes a protein ubiquitously expressed in eukaryotic cells. SNX2 contains a PX domain that binds phosphoinositides and a BAR (Bin/Amphiphysin/Rvs) domain responsible for membrane curvature sensing and tubulation. Together, these domains enable SNX2 to shape endosomal membranes and facilitate cargo vesicle formation. It also interacts with clathrin and dynamin to coordinate endocytic vesicle budding and recycling.

In cellular signaling, SNX2 regulates the trafficking of growth factor receptors such as EGFR, modulating receptor downregulation and signal duration. It contributes to Wnt pathway regulation by recycling Wntless, essential for ligand secretion. Disruption of SNX2 impairs endosome-to-Golgi transport, leading to accumulation of misrouted cargo and altered cell signaling. SNX2 has also been implicated in pathogen entry and immune receptor trafficking.

SNX2 antibody is widely used in membrane trafficking, signal transduction, and endocytosis research. It is suitable for immunoblotting, immunofluorescence, and subcellular localization studies to visualize endosomal compartments. In neurobiology, SNX2 detection supports studies of synaptic vesicle recycling and neurodegenerative disorders linked to retromer dysfunction. Its expression is also examined in cancer biology, where altered trafficking contributes to aberrant receptor signaling and invasion.

Structurally, SNX2 functions as a dimer that induces membrane tubulation and curvature through its BAR domain, while its PX domain anchors the complex to phosphoinositide-rich membranes. NSJ Bioreagents provides SNX2 antibody reagents validated for use in vesicle transport, endosomal sorting, and receptor recycling studies.

UniProt: O60749