NPLOC4 antibody (AA 1-510)

Catalog No. ABIN7869444

The Rabbit Polyclonal anti-NPLOC4 antibody is suitable to detect NPLOC4 in samples from Zebrafish (Danio rerio). It has been validated for IHC (p).

Quick Overview for NPLOC4 antibody (AA 1-510) (ABIN7869444)

Target: NPLOC4 (Nuclear Protein Localization 4 Homolog (NPLOC4))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPLOC4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NPLOC4 Antibody

Binding Specificity: AA 1-510

Purpose: Zebrafish Nploc4 Antibody / Npl4 / Nuclear protein localization protein 4

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Nploc4 recombinant protein (amino acids M1-C510) was used as the immunogen for the Zebrafish Nploc4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Nploc4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Nploc4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NPLOC4

Target: NPLOC4 (Nuclear Protein Localization 4 Homolog (NPLOC4))

Alternative Name: Nploc4

Background: Nploc4, or nuclear protein localization protein four homolog, is a highly conserved protein involved in protein degradation and cellular stress response pathways. It acts as a cofactor in the disassembly of polyubiquitinated protein complexes, particularly in cooperation with the ATPase p97 also known as VCP. Nploc4 plays an essential role in extracting ubiquitinated substrates from membranes and protein complexes to facilitate their degradation by the proteasome.

In zebrafish, Nploc4 is expressed in multiple tissues and is especially active in proliferative or metabolically active cells. The zebrafish Nploc4 protein is an ortholog of the human NPLOC4 gene. Both versions of the protein share strong sequence and functional conservation, indicating that zebrafish can serve as a reliable model for studying NPLOC4 related biology.

Functionally, Nploc4 is part of a complex with Ufd1 and p97, and this complex is critical for endoplasmic reticulum associated degradation, chromatin remodeling, and cell cycle regulation. Disruption of Nploc4 function in zebrafish leads to defects in protein homeostasis and developmental abnormalities, especially in systems that are sensitive to proteotoxic stress such as the nervous system and musculature.

Given its central role in maintaining cellular protein quality control and its conservation with human NPLOC4, zebrafish Nploc4 is commonly used in developmental studies and disease models related to protein aggregation, neurodegeneration, and cellular stress adaptation.

UniProt: Q7ZVI8