QRSL1 antibody (AA 1-528)

Catalog No. ABIN7869453

The Rabbit Polyclonal anti-QRSL1 antibody is suitable to detect QRSL1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for QRSL1 antibody (AA 1-528) (ABIN7869453)

Target: QRSL1 (Glutaminyl-tRNA Synthase (Glutamine-Hydrolyzing)-Like 1 (QRSL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This QRSL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-QRSL1 Antibody

Binding Specificity: AA 1-528

Purpose: QRSL1 Antibody / Glutamyl-tRNA amidotransferase subunit A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human QRSL1 recombinant protein (Position: M1-Q528) was used as the immunogen for the QRSL1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the QRSL1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the QRSL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for QRSL1

Target: QRSL1 (Glutaminyl-tRNA Synthase (Glutamine-Hydrolyzing)-Like 1 (QRSL1))

Alternative Name: QRSL1

Background: QRSL1 antibody detects Glutamyl-tRNA amidotransferase subunit A, a mitochondrial enzyme involved in the synthesis of correctly charged glutaminyl-tRNA required for mitochondrial protein translation. Encoded by the QRSL1 gene on chromosome 6q15, this protein forms part of the heterotrimeric GatCAB complex (comprising QRSL1, GATB, and GATC) that catalyzes the transamidation of misacylated Glu-tRNA(Gln) into Gln-tRNA(Gln). This reaction maintains translational fidelity in mitochondria, ensuring proper expression of the mitochondrial genome.

Structurally, QRSL1 is a 477-amino-acid mitochondrial matrix protein of approximately 53 kilodaltons containing a glutaminase domain and an amidotransferase catalytic domain that cooperatively enable amide group transfer from glutamine to tRNA-bound glutamate. It interacts with the GATB and GATC subunits to form a functional enzyme complex anchored to mitochondrial ribosomes. QRSL1 is ubiquitously expressed, with high levels in energy-demanding tissues such as heart, muscle, and brain.

The QRSL1 antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, mitochondrial translation accuracy, and metabolic regulation. Western blot analysis detects a 53 kilodalton band corresponding to QRSL1, while immunofluorescence reveals characteristic mitochondrial localization. This antibody serves as a reliable tool for evaluating mitochondrial translation machinery and enzyme complex formation.

Functionally, QRSL1 ensures accurate decoding of the mitochondrial genetic code by converting incorrectly charged tRNAs into their correct aminoacylated forms. Mutations in QRSL1 are linked to mitochondrial encephalopathy and lactic acidosis due to impaired oxidative phosphorylation and protein synthesis defects. The enzyme also contributes to overall mitochondrial proteostasis by preventing misincorporation of amino acids during translation. The QRSL1 antibody supports mechanistic studies of mitochondrial translation, respiratory chain biogenesis, and genetic disorders caused by tRNA modification defects. NSJ Bioreagents validates this antibody for its applications ensuring reliable detection in mitochondrial enzymatic studies.

UniProt: Q9H0R6