RARS2 antibody (AA 1-560)

Catalog No. ABIN7869474

The Rabbit Polyclonal anti-RARS2 antibody is suitable to detect RARS2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for RARS2 antibody (AA 1-560) (ABIN7869474)

Target: RARS2 (Arginyl-tRNA Synthetase 2, Mitochondrial (RARS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RARS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RARS2 Antibody

Binding Specificity: AA 1-560

Purpose: RARS2 Antibody / Arginyl-tRNA synthetase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RARS2 recombinant protein (Position: M1-R560) was used as the immunogen for the RARS2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RARS2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RARS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RARS2

Target: RARS2 (Arginyl-tRNA Synthetase 2, Mitochondrial (RARS2))

Alternative Name: RARS2

Background: RARS2 antibody detects Arginyl-tRNA synthetase 2, mitochondrial, an enzyme that catalyzes the attachment of arginine to its corresponding mitochondrial tRNA during protein synthesis. RARS2 plays a critical role in maintaining mitochondrial translation and respiratory chain function. The RARS2 antibody is used in mitochondrial biology, genetics, and neurodegenerative research to study mitochondrial protein synthesis and energy metabolism.

RARS2 is encoded by the RARS2 gene located on human chromosome 6q16.1. The protein is approximately 580 amino acids long and localizes to the mitochondrial matrix, where it contributes to the translation of 13 essential oxidative phosphorylation (OXPHOS) subunits encoded by mitochondrial DNA. RARS2 functions as part of the aminoacyl-tRNA synthetase family, ensuring fidelity of tRNA charging for mitochondrial translation.

The RARS2 antibody detects a 65 kilodalton protein by western blot and reveals mitochondrial localization under confocal imaging. RARS2 ensures accurate incorporation of arginine during protein synthesis, maintaining the stoichiometry of respiratory chain complexes. Loss of RARS2 activity disrupts mitochondrial protein translation, leading to decreased OXPHOS efficiency and increased oxidative stress.

Mutations in RARS2 cause pontocerebellar hypoplasia type 6, a neurodegenerative disorder characterized by developmental delay, seizures, and cerebellar atrophy. In cellular models, depletion of RARS2 impairs mitochondrial respiration, ATP production, and neuronal viability. Its regulation is closely tied to mitochondrial biogenesis and stress adaptation.

As an essential enzyme for mitochondrial translation and neural health, RARS2 is a valuable model for understanding mitochondrial gene expression and energy homeostasis. NSJ Bioreagents provides a validated RARS2 antibody optimized for its applications, supporting studies in mitochondrial translation, metabolic regulation, and neurodegenerative disease mechanisms.

UniProt: Q5T160