ME1 antibody (AA 1-572)

Catalog No. ABIN7869481

The Rabbit Polyclonal anti-ME1 antibody (ABIN7869481) specifically detects ME1 in WB, IF, IHC (p) and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ME1 antibody (AA 1-572) (ABIN7869481)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-ME1 Antibody

Binding Specificity: AA 1-572

Purpose: ME1 Antibody / Malic enzyme 1

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids M1-Q572) was used as the immunogen for the ME1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ME1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ME1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1

Background: ME1 (Malic enzyme 1) is a cytosolic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, producing NADPH in the process. This reaction plays a critical role in cellular metabolism by linking the tricarboxylic acid (TCA) cycle to lipid biosynthesis and antioxidant defense. ME1-derived NADPH is essential for fatty acid and cholesterol synthesis, as well as for maintaining redox balance in cells.

ME1 is expressed in various tissues, with particularly high levels in lipogenic tissues such as liver, adipose tissue, and lactating mammary gland. Its activity is regulated by nutritional and hormonal signals, including carbohydrate intake and insulin, making it a key player in metabolic adaptation. Due to its involvement in energy metabolism and biosynthetic processes, ME1 has been widely studied in the contexts of metabolic regulation and biochemical pathway integration.

The ME1 antibody is a valuable reagent for detecting ME1 in research applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the ME1 antibody from NSJ Bioreagents to assess tissue-specific expression, study its role in lipid metabolism, and investigate its contribution to NADPH production. With strong specificity and reproducible performance, the ME1 antibody supports a wide range of studies in cellular metabolism, enzymology, and biochemical research.

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha