Plastin 3 antibody (AA 1-580)

Catalog No. ABIN7869486

The Rabbit Polyclonal anti-Plastin 3 antibody is suitable to detect Plastin 3 in samples from Human, Mouse and Rat. It has been validated for WB, IP, ICC, ELISA, IF and FACS.

Quick Overview for Plastin 3 antibody (AA 1-580) (ABIN7869486)

Target: Plastin 3 (PLS3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plastin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Plastin 3 Antibody

Binding Specificity: AA 1-580

Purpose: PLS3 Antibody / Plastin 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLS3 recombinant protein (Position: M1-D580) was used as the immunogen for the PLS3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLS3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Plastin 3

Target: Plastin 3 (PLS3)

Alternative Name: PLS3

Background: PLS3 antibody detects Plastin-3, encoded by the PLS3 gene on chromosome Xq23. PLS3 antibody is widely used in research on actin dynamics, cytoskeletal regulation, and bone disease. Plastin-3 belongs to the plastin family of actin-bundling proteins, which regulate the organization of actin filaments in diverse cellular processes including adhesion, migration, endocytosis, and vesicle trafficking. PLS3 is expressed in many tissues, with enrichment in intestine, kidney, and bone, and is critical for cytoskeletal integrity and mechanosensing.

Structurally, Plastin-3 is a ~70 kDa protein containing two actin-binding domains and a regulatory calcium-binding EF-hand motif. The dual actin-binding domains enable crosslinking of actin filaments into bundles, while the EF-hand motif confers regulation by calcium signaling. Isoforms generated by alternative splicing provide tissue-specific modulation of activity.

Functionally, PLS3 stabilizes actin structures required for cell adhesion, vesicle trafficking, and mechanotransduction. In osteocytes, PLS3 contributes to sensing mechanical load and bone remodeling. In epithelial cells, it supports brush border formation and endocytic processes. Loss of function impairs cytoskeletal stability, leading to cellular fragility and disease phenotypes. Researchers use PLS3 antibody to study actin remodeling, mechanobiology, and cytoskeletal disorders.

Clinically, mutations in PLS3 are associated with X-linked osteoporosis, a rare bone fragility disorder characterized by low bone mineral density and fracture susceptibility. Variants in PLS3 impair actin bundling in osteocytes, disrupting mechanosensing and bone homeostasis. PLS3 has also been implicated in cancer, where altered expression influences invasion and metastasis. NSJ Bioreagents supplies PLS3 antibody for use in bone biology, cytoskeletal research, and oncology.

Experimentally, PLS3 antibody is applied in western blotting to detect the ~70 kDa protein, in immunofluorescence to visualize actin bundles, and in immunohistochemistry to study tissue distribution. Co-immunoprecipitation with PLS3 antibody identifies actin-binding partners and regulatory proteins. Functional studies using PLS3 antibody help link cytoskeletal organization with mechanical and signaling pathways.

UniProt: P13797