FAM48A/P38IP antibody (AA 1-713)

Catalog No. ABIN7869535

The Rabbit Polyclonal anti-FAM48A/P38IP antibody (ABIN7869535) specifically detects FAM48A/P38IP in WB, ELISA, IF and ICC. The antibody is reactive with Human and Rat samples.

Quick Overview for FAM48A/P38IP antibody (AA 1-713) (ABIN7869535)

Target: FAM48A/P38IP (SUPT20H) (Suppressor of Ty 20 Homolog (SUPT20H))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM48A/P38IP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-FAM48A/P38IP Antibody

Binding Specificity: AA 1-713

Purpose: SUPT20H Antibody / Transcription factor SPT20 homolog

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SUPT20H recombinant protein (Position: M1-Q713) was used as the immunogen for the SUPT20H antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SUPT20H antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SUPT20H antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FAM48A/P38IP

Target: FAM48A/P38IP (SUPT20H) (Suppressor of Ty 20 Homolog (SUPT20H))

Alternative Name: SUPT20H

Background: SUPT20H antibody detects Transcription factor SPT20 homolog, a multifunctional protein involved in transcriptional regulation, chromatin remodeling, and proteostasis. Encoded by the SUPT20H gene on chromosome 13q12.3, this evolutionarily conserved factor plays dual roles as a component of transcriptional coactivator complexes and as a regulator of protein homeostasis in the cytoplasm. SUPT20H participates in the regulation of RNA polymerase II-mediated transcription, chromatin accessibility, and cellular stress response.

Structurally, SUPT20H contains conserved coiled-coil and low-complexity regions that facilitate interactions with chromatin-associated proteins, transcription factors, and the proteasome. It is a functional homolog of yeast SPT20, a core subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) transcriptional coactivator complex that links histone acetylation to gene activation. In mammalian systems, SUPT20H contributes to nucleosome remodeling and transcriptional coactivation by coupling histone modification with recruitment of RNA polymerase II at promoter regions.

The SUPT20H antibody is widely used in epigenetics, gene regulation, and cell biology research to study transcriptional activation, chromatin remodeling, and stress adaptation. Western blot analysis detects a 100 kilodalton band corresponding to SUPT20H, while immunofluorescence reveals both nuclear and cytoplasmic staining, reflecting its multifunctional localization. This antibody enables researchers to investigate how chromatin dynamics and transcriptional machinery coordinate gene expression in response to cellular cues.

Beyond its nuclear role, SUPT20H interacts with the proteasome and heat shock proteins to regulate protein degradation and quality control under stress conditions. It assists in clearing misfolded proteins and maintaining cytoplasmic proteostasis. Dysregulation of SUPT20H expression or mutations affecting its protein-interaction motifs have been linked to altered transcriptional fidelity, stress sensitivity, and oncogenic transformation. The SUPT20H antibody provides a powerful tool for characterizing these processes at the molecular level. NSJ Bioreagents validates this antibody for its applications, ensuring dependable performance in transcriptional and proteostasis research.

UniProt: Q8NEM7