TDRD5 antibody (AA 1-722)

Catalog No. ABIN7869536

This Rabbit Polyclonal antibody specifically detects TDRD5 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TDRD5 antibody (AA 1-722) (ABIN7869536)

Target: TDRD5 (Tudor Domain Containing 5 (TDRD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDRD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TDRD5 Antibody

Binding Specificity: AA 1-722

Purpose: TDRD5 Antibody / Tudor domain-containing protein 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TDRD5 recombinant protein (Position: M1-E722) was used as the immunogen for the TDRD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TDRD5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TDRD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TDRD5

Target: TDRD5 (Tudor Domain Containing 5 (TDRD5))

Alternative Name: TDRD5

Background: TDRD5 antibody detects Tudor domain-containing protein 5, encoded by the TDRD5 gene. Tudor domain-containing protein 5 is a germ cell-specific protein that regulates piRNA pathway function, transposon silencing, and spermatogenesis. TDRD5 antibody provides researchers with a tool for studying reproductive biology, germline epigenetics, and fertility.

Tudor domain-containing protein 5 contains multiple Tudor domains that bind symmetrically dimethylated arginine residues on Piwi-interacting proteins and other components of the piRNA pathway. Research using TDRD5 antibody has shown that it localizes to nuage structures and intermitochondrial cement in germ cells, where it assembles piRNA-protein complexes. These complexes are critical for silencing transposable elements and protecting genome integrity in the germline.

Studies with TDRD5 antibody have revealed that knockout models lacking TDRD5 display impaired spermatogenesis, defective piRNA processing, and male infertility. This demonstrates its essential role in germ cell development. TDRD5 works in concert with other Tudor domain proteins, such as TDRD1 and TDRD9, to coordinate piRNA biogenesis and transposon repression.

Dysfunction of Tudor domain-containing protein 5 has been associated with infertility and germline genome instability. Research using TDRD5 antibody has shown that disruption of piRNA regulation allows mobilization of retrotransposons, resulting in DNA damage and germ cell loss. These findings emphasize its protective function in the germline.

TDRD5 antibody is widely applied in immunohistochemistry, immunofluorescence, and western blotting. Immunohistochemistry reveals expression in testis, immunofluorescence demonstrates localization in nuage and germ granules, and western blotting quantifies protein levels during germ cell development. These methods make TDRD5 antibody indispensable in reproductive biology research.

By supplying validated TDRD5 antibody reagents, NSJ Bioreagents supports studies into germline epigenetics, piRNA pathways, and fertility. Detection of Tudor domain-containing protein 5 provides researchers with insight into how germ cell proteins maintain genome stability and reproductive capacity.

UniProt: Q8NAT2

Pathways: Ribonucleoprotein Complex Subunit Organization