SIK1 antibody (AA 1-783)

Catalog No. ABIN7869556

The Rabbit Polyclonal anti-SIK1 antibody (ABIN7869556) specifically detects SIK1 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIK1 antibody (AA 1-783) (ABIN7869556)

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SIK1 Antibody

Binding Specificity: AA 1-783

Purpose: SIK1 Antibody / Salt-inducible kinase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Snf1lk/SIK1 recombinant protein (Position: M1-Q783) was used as the immunogen for the SIK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SIK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SIK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SIK1

Target: SIK1 (Salt-Inducible Kinase 1 (SIK1))

Alternative Name: SIK1

Background: SIK1 antibody detects Salt-inducible kinase 1, a serine/threonine kinase that regulates energy metabolism, stress signaling, and transcriptional responses to hormonal cues. SIK1 belongs to the AMP-activated protein kinase (AMPK) family and is activated by LKB1-mediated phosphorylation. The SIK1 antibody is commonly used to investigate glucose metabolism, circadian rhythm, and stress adaptation in mammalian cells.

SIK1 is encoded by the SIK1 gene located on human chromosome 21q22.3. The protein is approximately 78 kilodaltons and consists of an N-terminal kinase domain, an autoinhibitory sequence, and a C-terminal region rich in phosphorylation sites. SIK1 modulates transcription by phosphorylating CREB-regulated transcription coactivators (CRTCs), leading to their cytoplasmic retention and suppression of CREB-dependent gene expression.

The SIK1 antibody detects a strong 85 kilodalton band by western blot. Under stress or hormonal stimulation, SIK1 undergoes rapid induction and nuclear translocation. It acts as a feedback regulator controlling CREB activity, gluconeogenic gene expression, and circadian rhythm stability.

Functionally, SIK1 plays an important role in metabolic homeostasis. It regulates hepatic glucose output, lipid metabolism, and skeletal muscle adaptation to exercise. In the brain, SIK1 contributes to neuronal plasticity and sleep-wake regulation. Dysregulation of SIK1 expression or phosphorylation has been implicated in hypertension, metabolic syndrome, and mood disorders.

SIK1 is a key mediator of hormonal signaling via cAMP and glucocorticoids. It interacts with 14-3-3 proteins and phosphatases, allowing dynamic control of kinase activity in response to changing energy states. NSJ Bioreagents provides a validated SIK1 antibody optimized for its applications, supporting exploration of metabolic regulation, transcriptional control, and stress physiology.

UniProt: P57059

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process