N4BP1 antibody (AA 1-885)

Catalog No. ABIN7869602

This Rabbit Polyclonal antibody specifically detects N4BP1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for N4BP1 antibody (AA 1-885) (ABIN7869602)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-N4BP1 Antibody

Binding Specificity: AA 1-885

Purpose: N4BP1 Antibody / NEDD4-binding protein 1 / KIAA0615

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids M1-K885) was used as the immunogen for the N4BP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the N4BP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the N4BP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73?, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73? for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73? and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

UniProt: O75113