SUPT16H antibody (AA 1-926)

Catalog No. ABIN7869627

The Rabbit Polyclonal anti-SUPT16H antibody is suitable to detect SUPT16H in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IF, ICC and FACS.

Quick Overview for SUPT16H antibody (AA 1-926) (ABIN7869627)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SUPT16H Antibody

Binding Specificity: AA 1-926

Purpose: SUPT16H Antibody / Suppressor of Ty 16

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SUPT16H recombinant protein (Position: M1-E926) was used as the immunogen for the SUPT16H antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SUPT16H antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SUPT16H antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background: SUPT16H antibody detects Suppressor of Ty 16 homolog, a chromatin-associated protein that forms part of the FACT (facilitates chromatin transcription) complex. Together with SSRP1, SUPT16H reorganizes nucleosomes during transcription, replication, and DNA repair. The SUPT16H antibody is frequently used in chromatin biology and gene-expression studies to examine histone dynamics and transcriptional regulation.

SUPT16H is encoded by the SUPT16H gene located on human chromosome 14q11.2. The protein is approximately 120 kilodaltons and consists of an N-terminal dimerization domain, a middle domain that binds histones H2A-H2B, and a C-terminal acidic region responsible for interactions with RNA polymerase II and other transcriptional regulators. Through its role in nucleosome disassembly and reassembly, SUPT16H maintains chromatin integrity during active transcription.

A SUPT16H antibody detects a 120 kilodalton band in western blot assays and demonstrates strong nuclear localization by immunofluorescence. FACT complex activity is essential for the passage of polymerases through chromatin and the preservation of epigenetic marks. SUPT16H regulates promoter accessibility and prevents inappropriate histone eviction, balancing gene activation with chromatin stability.

Altered SUPT16H function affects transcription elongation, replication stress response, and genome maintenance. Viral proteins such as HIV Tat exploit FACT to facilitate viral transcription, while suppression of SUPT16H reduces viral replication. Overexpression is observed in several cancers, where it supports rapid proliferation by maintaining chromatin fluidity and preventing DNA damage-induced senescence.

Because of its pivotal role in chromatin dynamics, SUPT16H serves as a potential target for cancer therapy and antiviral drug development. NSJ Bioreagents provides a validated SUPT16H antibody for its applications, supporting research into transcriptional control, epigenetic remodeling, and genome integrity.

UniProt: Q9Y5B9

Pathways: Chromatin Binding