Recombinant CSTA antibody (AA 1-98)

Catalog No. ABIN7869662

This Rabbit Monoclonal antibody specifically detects CSTA in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant CSTA antibody (AA 1-98) (ABIN7869662)

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CSTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: CSTA-9087R

Product Details anti-CSTA Antibody

Binding Specificity: AA 1-98

Purpose: Recombinant Cystatin A Antibody / CSTA (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant fragment of human CSTA protein (within amino acids 1-98) was used as the immunogen for the recombinant Cystatin A antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1.Optimal dilution of the recombinant Cystatin A antibody should be determined by the researcher.

2. This recombinant Cystatin A antibody was recombinantly expressed in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the recombinant Cystatin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CSTA

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Alternative Name: Cystatin A

Background: Cystatin A antibody targets Cystatin A, a cytoplasmic cysteine protease inhibitor encoded by the CSTA gene and a member of the type I cystatin family. Cystatin A is also widely known as Stefin A and functions as an endogenous inhibitor of papain-like cysteine proteases, including cathepsins B, H, and L. By regulating intracellular protease activity, Cystatin A plays an essential role in protecting epithelial cells from excessive proteolysis and maintaining tissue integrity.

Cystatin A is predominantly localized in the cytoplasm and is highly expressed in stratified squamous epithelia such as epidermis, oral mucosa, esophagus, and cervix. It is closely associated with epithelial differentiation and keratinization processes, where controlled protease inhibition is required for proper barrier formation. Cystatin A antibody detection is therefore useful for studying epithelial maturation, protease balance, and barrier-related biology.

Functionally, Cystatin A binds tightly to cysteine proteases and suppresses their enzymatic activity, thereby influencing cellular processes including apoptosis, inflammation, and cell migration. Through modulation of cathepsin activity, Cystatin A contributes to controlled protein turnover and cellular homeostasis. CSTA antibody reagents support research into protease-inhibitor networks that are critical for normal epithelial function and stress responses.

Altered expression of Cystatin A has been reported in multiple disease contexts, particularly in epithelial-derived cancers. Reduced or heterogeneous CSTA expression has been associated with increased invasiveness and poor differentiation, while strong expression is often retained in well-differentiated squamous cell carcinomas. Changes in Cystatin A levels have also been linked to inflammatory skin disorders and barrier dysfunction, highlighting its relevance in both cancer biology and dermatological research.

Clone CSTA/9087R is designed to recognize Cystatin A in research applications. Cystatin A antibody reagents are suitable for detecting protein expression and localization in epithelial tissues and tumor samples, supporting investigations into protease regulation, epithelial differentiation, and disease-associated alterations in cysteine protease inhibition.

UniProt: P01040

Pathways: Response to Water Deprivation