NCAPG antibody (AA 1-993)

Catalog No. ABIN7869671

This Rabbit Polyclonal antibody specifically detects NCAPG in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for NCAPG antibody (AA 1-993) (ABIN7869671)

Target: NCAPG (Non-SMC Condensin I Complex, Subunit G (NCAPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NCAPG Antibody

Binding Specificity: AA 1-993

Purpose: NCAPG Antibody / Non-SMC condensin I complex subunit G

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NCAPG recombinant protein (Position: M1-R993) was used as the immunogen for the NCAPG antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NCAPG antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NCAPG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NCAPG

Target: NCAPG (Non-SMC Condensin I Complex, Subunit G (NCAPG))

Alternative Name: NCAPG

Background: NCAPG antibody detects Non-SMC condensin I complex subunit G, an essential component of the condensin I complex that mediates chromosome condensation and segregation during mitosis. The UniProt recommended name is Non-SMC condensin I complex subunit G (NCAPG), with alternate names condensin complex subunit G, chromosome-associated protein G, and CAP-G. NCAPG functions as a regulatory subunit within the condensin complex, coordinating chromosome supercoiling and stabilization necessary for accurate chromosomal segregation.

During cell division, NCAPG antibody identifies a protein critical for organizing chromatin architecture. NCAPG, together with SMC2, SMC4, NCAPH, and NCAPD2, composes condensin I, which compacts chromatin fibers into mitotic chromosomes. It binds ATP-dependent SMC core units and interacts with regulatory partners that modulate DNA topology and cohesion release. In addition to its structural role, NCAPG has been linked to transcriptional regulation, genomic stability, and cell proliferation pathways. Abnormal expression of NCAPG correlates with aneuploidy, mitotic defects, and tumor progression in various cancers, including hepatocellular carcinoma, glioblastoma, and lung cancer.

The NCAPG antibody is used to monitor cell cycle progression, particularly in the G2/M phase where condensin activation occurs. Knockdown of NCAPG leads to chromosome segregation errors, DNA damage, and mitotic arrest. Studies also show NCAPG involvement in DNA repair and replication stress responses. The NCAPG gene is located on chromosome 4p15.32 and encodes a 1015-amino acid nuclear protein. NCAPG's expression peaks in proliferating cells and declines upon differentiation, making it a useful marker of mitotic activity.

Beyond its canonical mitotic role, NCAPG contributes to chromatin reorganization during transcriptional activation and may interact with epigenetic regulators. Upregulation of NCAPG in cancer promotes oncogenic growth via activation of PI3K/AKT and MAPK signaling pathways. High NCAPG levels are associated with poor prognosis and therapeutic resistance, suggesting it as a biomarker and target for anti-proliferative therapy. Researchers use NCAPG antibody for western blotting, immunostaining, and flow cytometry to study mitotic chromosome structure and cell cycle regulation. NSJ Bioreagents offers validated reagents for detecting human NCAPG to support research in cell division and chromosomal biology.

UniProt: Q9BPX3