ARHGEF12 antibody (AA 10-1426)

Catalog No. ABIN7869682

The Rabbit Polyclonal anti-ARHGEF12 antibody (ABIN7869682) specifically detects ARHGEF12 in ELISA, WB, IF, IP, IHC (p) and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ARHGEF12 antibody (AA 10-1426) (ABIN7869682)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-ARHGEF12 Antibody

Binding Specificity: AA 10-1426

Purpose: ARHGEF12 Antibody / LARG

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids 10-1426) was used as the immunogen for the ARHGEF12 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ARHGEF12 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ARHGEF12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: ARHGEF12

Background: ARHGEF12, also known as leukemia-associated Rho guanine nucleotide exchange factor or LARG, is a member of the RhoGEF family involved in the regulation of Rho GTPases. These small signaling proteins are crucial for controlling a variety of cellular processes, including cytoskeletal dynamics, cell polarity, migration, and transcriptional activity. ARHGEF12 acts as a guanine nucleotide exchange factor that specifically activates RhoA, linking it to G protein-coupled receptor signaling pathways and other upstream stimuli.

ARHGEF12 is widely expressed in multiple tissue types and has been implicated in various physiological and pathological conditions, such as embryonic development, wound healing, and cancer progression. Alternative splicing of the ARHGEF12 gene gives rise to several isoforms that may vary in function and cellular localization, making precise detection and characterization essential in research.

A high-quality ARHGEF12 antibody is a critical tool for investigating the diverse roles of this protein in cellular signaling and disease. The ARHGEF12 antibody can be used in western blotting to evaluate protein levels, in immunofluorescence to assess subcellular localization, and in immunohistochemistry for tissue-specific expression profiling. NSJ Bioreagents offers an extensively validated ARHGEF12 antibody designed to provide specificity and reproducibility across multiple experimental platforms.

UniProt: Q9NZN5

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling