VCP antibody (AA 10-512)

Catalog No. ABIN7869710

This Rabbit Polyclonal antibody specifically detects VCP in WB and IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for VCP antibody (AA 10-512) (ABIN7869710)

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VCP Antibody

Binding Specificity: AA 10-512

Purpose: Zebrafish Cdc48 Antibody / Valosin-containing protein / Vcp

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Cdc48 recombinant protein (amino acids D10-K512) was used as the immunogen for the Zebrafish Cdc48 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Cdc48 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Cdc48 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: Cdc48

Background: Zebrafish Cdc48, also known as cell division cycle 48, is a crucial ATPase that is involved in various cellular processes including protein degradation, cell cycle regulation, and membrane dynamics. Cdc48 is part of the AAA+ ATPase family and functions as a part of the 26S proteasome pathway, where it helps to extract polyubiquitinated substrates from the proteasome or other cellular compartments for degradation. Additionally, Cdc48 plays a role in endoplasmic reticulum associated degradation (ERAD), maintaining cellular homeostasis by ensuring the removal of misfolded proteins.

Zebrafish Cdc48 is an ortholog of the human CDC48 protein, also known as p97. It shares significant sequence and functional conservation with its human counterpart, ensuring that it performs similar roles in regulating protein turnover, signaling pathways, and cell cycle progression. Like human p97, zebrafish Cdc48 is essential for the regulation of processes such as DNA repair, organelle biogenesis, and apoptosis, and its dysfunction can result in various diseases, including neurodegenerative disorders and certain cancers.

The use of a Zebrafish Cdc48 antibody is an invaluable tool for studying protein quality control mechanisms and their role in cellular stress responses. Such antibodies are essential for applications including western blot, immunohistochemistry, immunofluorescence, and ELISA. With a Zebrafish Cdc48 antibody, researchers can analyze Cdc48 expression in different tissues, study its involvement in proteostasis, and investigate how alterations in its function impact development and disease.

Currently, no isoforms of zebrafish Cdc48 have been documented, but its essential role in cellular maintenance and its conservation across species make it an important target for research. A high-quality Zebrafish Cdc48 antibody provides essential insights into cellular stress mechanisms and the regulation of the ubiquitin-proteasome system in zebrafish models.

UniProt: Q7ZU99

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway