SASS6 antibody (AA 101-657)

Catalog No. ABIN7869900

The Rabbit Polyclonal anti-SASS6 antibody is suitable to detect SASS6 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and FACS.

Quick Overview for SASS6 antibody (AA 101-657) (ABIN7869900)

Target: SASS6 (Spindle Assembly 6 Homolog (SASS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SASS6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SASS6 Antibody

Binding Specificity: AA 101-657

Purpose: SASS6 Antibody / Spindle assembly abnormal protein 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SASS6 recombinant protein (Position: E101-S657) was used as the immunogen for the SASS6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SASS6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SASS6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SASS6

Target: SASS6 (Spindle Assembly 6 Homolog (SASS6))

Alternative Name: SASS6

Background: SASS6 antibody recognizes Spindle assembly abnormal protein 6 homolog, a centriolar protein required for centriole duplication and proper cell division. Encoded by the SASS6 gene on chromosome 1p21.2, this protein is a structural component of the cartwheel, a ninefold symmetric scaffold that initiates centriole assembly. SASS6 self-oligomerizes through its coiled-coil domain to establish the radial symmetry of nascent centrioles, ensuring accurate duplication and inheritance of centrosomes during each cell cycle. The protein localizes to the proximal ends of centrioles and is essential for maintaining centrosome integrity and mitotic spindle organization.

Loss of SASS6 disrupts centriole formation, resulting in monopolar spindles and abnormal cell division. Mutations in SASS6 cause primary microcephaly, characterized by reduced brain size and defective neural progenitor proliferation. The SASS6 antibody allows detection of centriolar structures in cultured cells and tissue samples. Immunofluorescence using this antibody reveals punctate staining at centrosomes during interphase and mitosis, while western blotting identifies a 65 kilodalton band. Because of its critical role in centrosome biogenesis, SASS6 serves as a core marker for centriole assembly studies and cell cycle regulation research.

Beyond its role in structural assembly, SASS6 interacts with STIL and CEP135 to coordinate recruitment of microtubule-nucleating proteins. Its regulated degradation through the APC/C complex ensures centriole duplication occurs only once per cell cycle. Overexpression of SASS6 leads to centrosome amplification, a phenotype frequently observed in cancer cells. The SASS6 antibody is thus valuable for examining centrosome abnormalities and chromosomal instability associated with tumorigenesis. Researchers also employ this antibody in developmental studies to trace centriole formation during early embryogenesis. NSJ Bioreagents provides a validated SASS6 antibody for use in immunofluorescence, western blot, and cell imaging assays focused on centrosome dynamics and mitotic control.

UniProt: Q6UVJ0