MAP1B antibody (AA 102-1849)

Catalog No. ABIN7869909

The Rabbit Polyclonal anti-MAP1B antibody is suitable to detect MAP1B in samples from Human, Mouse and Rat. It has been validated for IHC, WB, ELISA, FACS and IF.

Quick Overview for MAP1B antibody (AA 102-1849) (ABIN7869909)

Target: MAP1B (Microtubule-Associated Protein 1B (MAP1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MAP1B Antibody

Binding Specificity: AA 102-1849

Purpose: MAP1B Antibody / Microtubule-associated protein 1B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MAP1B recombinant protein (Position: H102-R1849) was used as the immunogen for the MAP1B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MAP1B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MAP1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MAP1B

Target: MAP1B (Microtubule-Associated Protein 1B (MAP1B))

Alternative Name: MAP1B

Background: MAP1B antibody detects Microtubule-associated protein 1B, a neuronal cytoskeletal protein that regulates microtubule stability, axon guidance, and neuronal differentiation. The UniProt recommended name is Microtubule-associated protein 1B (MAP1B). This large microtubule-binding protein plays an essential role during nervous system development by stabilizing and organizing microtubule networks within axons and dendrites.

Functionally, MAP1B antibody identifies a precursor protein of approximately 2,460 amino acids that is proteolytically cleaved into heavy and light chains. These subunits assemble with microtubules and actin filaments, promoting cytoskeletal crosslinking. MAP1B is enriched in developing neurons, where it modulates growth cone dynamics and neurite outgrowth, influencing axonal pathfinding and regeneration.

The MAP1B gene is located on chromosome 5q13.2 and is highly expressed in the central and peripheral nervous systems. MAP1B associates with other neuronal cytoskeletal proteins, such as tau and MAP2, to regulate polymerization and microtubule stability. Phosphorylation of MAP1B by kinases including GSK3 and CDK5 modulates its binding affinity and spatial localization, fine-tuning neuronal polarity and maturation.

Pathologically, aberrant MAP1B expression or phosphorylation is associated with neurodevelopmental and neurodegenerative diseases, including Alzheimeri 1/2s and Parkinsoni 1/2s disease. In tumor cells, MAP1B re-expression correlates with altered cytoskeletal organization and invasiveness. Research using MAP1B antibody supports studies in axonal transport, cytoskeletal remodeling, and neuronal repair mechanisms.

MAP1B antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect neuronal microtubule-associated proteins. NSJ Bioreagents offers MAP1B antibody reagents optimized for neurobiology, cytoskeletal, and developmental research applications.

Structurally, Microtubule-associated protein 1B contains multiple microtubule-binding domains and an actin-binding region, facilitating cytoskeletal integration. Its dynamic phosphorylation pattern determines its localization and activity during neuron growth and regeneration. This antibody provides a reliable tool to examine MAP1B's regulatory functions in cytoskeletal organization and axonal development.

UniProt: P46821

Pathways: Microtubule Dynamics, Regulation of Cell Size