AQP2 antibody (AA 102-271)

Catalog No. ABIN7869912

The Rabbit Polyclonal anti-AQP2 antibody is suitable to detect AQP2 in samples from Rat and Mouse. It has been validated for WB, ELISA and FACS.

Quick Overview for AQP2 antibody (AA 102-271) (ABIN7869912)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-AQP2 Antibody

Binding Specificity: AA 102-271

Purpose: Aqp2 Antibody / Aquaporin 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271) was used as the immunogen for the Aqp2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Aqp2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Aqp2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aqp2

Background: The AQP2 antibody targets Aquaporin-2, a water channel protein encoded by the AQP2 gene. Aquaporin-2 is an essential component of the kidney's water reabsorption system, located in the apical membrane of collecting duct principal cells. It mediates vasopressin-regulated water transport, thereby controlling urine concentration and maintaining body fluid balance. The AQP2 antibody enables researchers to study renal physiology, osmoregulation, and disorders of water homeostasis such as diabetes insipidus.

Aquaporin-2 belongs to the aquaporin family of integral membrane proteins that selectively transport water across biological membranes. Under the influence of the antidiuretic hormone vasopressin, AQP2-containing vesicles are trafficked to and fused with the apical plasma membrane, increasing water permeability. The AQP2 antibody provides a reliable means to visualize this regulated translocation process, making it a key tool for examining vasopressin signaling and collecting duct responsiveness.

Mutations in the AQP2 gene lead to nephrogenic diabetes insipidus (NDI), characterized by excessive urine production and an inability to concentrate urine despite normal vasopressin levels. The AQP2 antibody supports research into this condition by enabling detection of defective or mislocalized Aquaporin-2 in renal tissue. Experimental models show that AQP2 deficiency disrupts water retention and contributes to dehydration, underscoring its clinical relevance in renal water management.

Beyond kidney physiology, Aquaporin-2 has been detected in reproductive tissues and the central nervous system, suggesting broader roles in fluid transport and homeostasis. The AQP2 antibody supports such investigations, allowing comparative expression analysis across tissues and experimental conditions. Regulation of AQP2 expression and trafficking involves multiple pathways including cyclic AMP, protein kinase A, and ubiquitination-dependent endocytosis.

The AQP2 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, producing distinct apical membrane staining in kidney tissue. NSJ Bioreagents provides this antibody with verified specificity and reproducibility for renal physiology, endocrinology, and molecular signaling research. By enabling precise detection of Aquaporin-2, the AQP2 antibody advances understanding of water transport regulation and the molecular mechanisms underlying disorders of water balance.

UniProt: P56402

Pathways: Response to Water Deprivation