CTR9 antibody (AA 1022-1056)

Catalog No. ABIN7869922

The Rabbit Polyclonal anti-CTR9 antibody (ABIN7869922) specifically detects CTR9 in WB, IHC (p) and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for CTR9 antibody (AA 1022-1056) (ABIN7869922)

Target: CTR9 (RNA Polymerase-Associated Protein CTR9 Homolog (CTR9))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-CTR9 Antibody

Binding Specificity: AA 1022-1056

Purpose: Ctr9 Antibody

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 1022-1056 from the mouse protein was used as the immunogen for the Ctr9 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Ctr9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Ctr9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CTR9

Target: CTR9 (RNA Polymerase-Associated Protein CTR9 Homolog (CTR9))

Alternative Name: Ctr9

Background: RNA polymerase-associated protein CTR9 homolog is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys- 4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway, may regulate DNA-association of STAT3.

UniProt: Q62018

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance