PLBD2 antibody (AA 108-525)

Catalog No. ABIN7870033

The Rabbit Polyclonal anti-PLBD2 antibody (ABIN7870033) specifically detects PLBD2 in ELISA, WB, IHC, FACS, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for PLBD2 antibody (AA 108-525) (ABIN7870033)

Target: PLBD2 (Phospholipase B Domain Containing 2 (PLBD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLBD2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PLBD2 Antibody

Binding Specificity: AA 108-525

Purpose: PLBD2 Antibody / Phospholipase B domain-containing protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLBD2 recombinant protein (Position: Q108-Q525) was used as the immunogen for the PLBD2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLBD2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLBD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PLBD2

Target: PLBD2 (Phospholipase B Domain Containing 2 (PLBD2))

Alternative Name: PLBD2

Background: PLBD2 antibody detects Phospholipase B domain-containing protein 2, a lysosomal enzyme implicated in lipid metabolism and cellular membrane remodeling. The UniProt recommended name is Phospholipase B domain-containing protein 2 (PLBD2). This protein belongs to the phospholipase B-like superfamily, which hydrolyzes both acyl chains of phospholipids to generate free fatty acids and glycerophosphates. Although the enzymatic activity of PLBD2 is still under characterization, evidence suggests roles in lipid turnover, immune defense, and autophagy.

Functionally, PLBD2 antibody recognizes a 516-amino-acid protein localized primarily in lysosomes and the endoplasmic reticulum. PLBD2 contains a conserved phospholipase B-like catalytic domain, which may mediate hydrolysis of membrane phospholipids and lysophospholipids. By modulating lipid composition, PLBD2 contributes to vesicle formation, endosomal trafficking, and lipid droplet metabolism. Its expression is induced by inflammatory cytokines and lipid stress, indicating a regulatory role in lipid homeostasis and immune signaling.

The PLBD2 gene is located on chromosome 12q13.13 and encodes a protein that shares structural features with classical phospholipases but displays distinct substrate specificity. While its catalytic mechanism remains partially defined, studies suggest that PLBD2 may participate in lipid recycling and detoxification under stress conditions. Proteomic analyses have identified PLBD2 as part of the lysosomal proteome, implicating it in membrane degradation and recycling of lipid components.

In immunology, PLBD2 has been linked to macrophage activation and inflammatory signaling. It may function as a lipid-modifying enzyme that influences phagosome maturation and pathogen clearance. Dysregulation of PLBD2 expression has been observed in metabolic disorders and cancers, suggesting that it plays a broader role in cellular metabolism and immune modulation. Elevated PLBD2 levels correlate with altered phospholipid profiles and increased autophagic activity in response to nutrient deprivation.

PLBD2 antibody is employed in lipidomics, immunology, and cell biology studies to investigate lysosomal function and lipid signaling pathways. It is useful for immunoblotting, immunofluorescence, and proteomic profiling of organelle-specific proteins. The enzyme's localization in the lysosomal compartment makes it an important marker for studies of membrane dynamics and lipid degradation. NSJ Bioreagents provides PLBD2 antibody reagents validated for use in research on lipid metabolism, autophagy, and membrane trafficking.

Structurally, PLBD2 contains the conserved GXGXXG motif characteristic of lipid-hydrolyzing enzymes and a putative signal peptide for targeting to intracellular membranes. Its domain organization suggests roles in lipid recognition and hydrolysis. Post-translational regulation may involve glycosylation and proteolytic processing that modulate catalytic efficiency. Ongoing research continues to clarify its enzymatic specificity and physiological relevance in lipid remodeling.

UniProt: Q8NHP8