ACSL5 antibody (AA 108-683)

Catalog No. ABIN7870035

The Rabbit Polyclonal anti-ACSL5 antibody is suitable to detect ACSL5 in samples from Human, Rat and Mouse. It has been validated for ELISA, WB and FACS.

Quick Overview for ACSL5 antibody (AA 108-683) (ABIN7870035)

Target: ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5 (ACSL5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSL5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ACSL5 Antibody

Binding Specificity: AA 108-683

Purpose: ACSL5 Antibody / Long chain fatty acid CoA ligase 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ACSL5 recombinant protein (Position: Y108-D683) was used as the immunogen for the ACSL5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ACSL5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ACSL5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ACSL5

Target: ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5 (ACSL5))

Alternative Name: ACSL5

Background: ACSL5 antibody detects Long chain fatty acid CoA ligase 5, an enzyme that activates long chain fatty acids for lipid metabolism, beta oxidation, and membrane synthesis. Encoded by the ACSL5 gene on chromosome 10q25.2, this enzyme belongs to the acyl CoA synthetase family, which catalyzes the conversion of free fatty acids to fatty acyl CoA thioesters using ATP and Coenzyme A. ACSL5 functions as a metabolic gatekeeper, determining whether lipids are directed toward energy production or anabolic pathways such as triglyceride and phospholipid synthesis.

Structurally, ACSL5 is an approximately 76 kilodalton enzyme localized to the outer mitochondrial membrane and the endoplasmic reticulum. It contains conserved AMP binding and acyl adenylate forming motifs required for catalysis of long chain fatty acids, typically those containing 12 to 20 carbon atoms. ACSL5 expression is highest in metabolically active tissues such as the small intestine, liver, and brown adipose tissue, where rapid fatty acid activation supports energy production during fasting, thermogenesis, and lipid absorption.

The ACSL5 antibody is widely used in metabolism, mitochondrial biology, and lipid biochemistry research to study fatty acid activation, transport, and oxidation. Western blot analysis detects a 76 kilodalton band corresponding to ACSL5, while immunofluorescence reveals perinuclear and mitochondrial membrane localization. This antibody provides a dependable tool for assessing lipid metabolic flux, energy homeostasis, and fatty acid utilization under physiological and pathological conditions.

Functionally, ACSL5 determines the metabolic fate of long chain fatty acids by channeling activated fatty acyl CoA into either mitochondrial beta oxidation for ATP production or endoplasmic reticulum based lipid synthesis. It interacts with key metabolic regulators such as carnitine palmitoyltransferase 1 (CPT1) and peroxisome proliferator activated receptor alpha (PPAR alpha), coordinating lipid oxidation and storage. Dysregulation of ACSL5 expression has been linked to obesity, insulin resistance, and nonalcoholic fatty liver disease, where altered fatty acid channeling contributes to lipid accumulation and oxidative stress. In intestinal epithelial cells, ACSL5 also plays a role in apoptosis regulation, reflecting its broader function in energy balance and cell survival.

Beyond energy metabolism, ACSL5 participates in mitochondrial dynamics and lipid signaling by influencing the composition of phospholipids that form mitochondrial membranes. Its activity affects membrane curvature and permeability, which can impact mitochondrial fission and fusion processes. Increased ACSL5 expression under stress conditions may enhance beta oxidation and reactive oxygen species production, whereas reduced expression limits fatty acid utilization and leads to metabolic inflexibility. The ACSL5 antibody allows detailed exploration of these adaptive responses and facilitates research into metabolic disorders, energy regulation, and mitochondrial health.

NSJ Bioreagents provides a validated ACSL5 antibody optimized for western blotting, immunohistochemistry, and immunofluorescence. This antibody enables sensitive and specific detection of Long chain fatty acid CoA ligase 5 across multiple tissues and experimental systems, supporting studies in lipid metabolism, energy signaling, and mitochondrial physiology.

UniProt: Q9ULC5