STK16 antibody (AA 109-268)

Catalog No. ABIN7870054

The Rabbit Polyclonal anti-STK16 antibody (ABIN7870054) specifically detects STK16 in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for STK16 antibody (AA 109-268) (ABIN7870054)

Target: STK16 (serine/threonine Kinase 16 (STK16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-STK16 Antibody

Binding Specificity: AA 109-268

Purpose: STK16 Antibody / Serine/threonine-protein kinase 16

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human STK16 recombinant protein (Position: E109-L268) was used as the immunogen for the STK16 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the STK16 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the STK16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for STK16

Target: STK16 (serine/threonine Kinase 16 (STK16))

Alternative Name: STK16

Background: STK16 antibody detects Serine/threonine-protein kinase 16, a membrane-associated kinase that regulates secretion, Golgi organization, and intracellular trafficking. The UniProt recommended name is Serine/threonine-protein kinase 16 (STK16). This constitutively active kinase associates with the Golgi apparatus and modulates vesicular transport between the Golgi and plasma membrane.

Functionally, STK16 antibody identifies a 305-amino-acid protein with a typical serine/threonine kinase domain and a C-terminal myristoylation site that anchors it to membranes. STK16 phosphorylates various substrates involved in exocytosis, including components of the SNARE complex. It is essential for maintaining Golgi structure, regulating secretion, and coordinating protein sorting within the secretory pathway.

The STK16 gene is located on chromosome 2q31.1 and is ubiquitously expressed, with enrichment in liver, pancreas, and secretory epithelial tissues. STK16i 1/2s kinase activity is regulated by autophosphorylation and membrane association. It also participates in cell cycle progression and polarized transport, suggesting broader roles in organelle biogenesis and signal transduction.

Pathologically, aberrant STK16 function has been implicated in tumorigenesis, metabolic disease, and Golgi stress. Overexpression may enhance secretion and proliferation, while inhibition disrupts vesicular trafficking and protein glycosylation. Research using STK16 antibody contributes to understanding kinase-mediated control of membrane traffic and its implications in disease and cellular physiology.

STK16 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to visualize Golgi-associated kinases and phosphorylation-dependent trafficking. NSJ Bioreagents offers STK16 antibody reagents optimized for studies of secretion, Golgi function, and signal transduction.

Structurally, STK16 belongs to the NAK (Numb-associated kinase) family and features an N-lobe typical of protein kinases, with regulatory elements that stabilize its constitutive activity. This antibody supports detailed characterization of STK16's role in kinase signaling and Golgi organization.

UniProt: O75716