Syncoilin antibody (AA 11-465)

Catalog No. ABIN7870085

This Rabbit Polyclonal antibody specifically detects Syncoilin in WB, ELISA and FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Syncoilin antibody (AA 11-465) (ABIN7870085)

Target: Syncoilin (Sync)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syncoilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Syncoilin Antibody

Binding Specificity: AA 11-465

Purpose: Syncoilin Antibody / Sync

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse Syncoilin/Sync recombinant protein (Position: D11-Q465) was used as the immunogen for the Syncoilin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Syncoilin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Syncoilin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Syncoilin

Target: Syncoilin (Sync)

Alternative Name: Syncoilin

Background: Syncoilin antibody detects Syncoilin, encoded by the SYNC gene on chromosome 1p34.1. Syncoilin antibody is widely used to study this intermediate filament protein that links the dystrophin-associated protein complex (DAPC) to the cytoskeleton. Syncoilin is a muscle-specific protein expressed in skeletal and cardiac muscle, where it stabilizes sarcolemmal structures and contributes to muscle integrity. By anchoring desmin intermediate filaments to the DAPC, Syncoilin provides structural support essential for force transmission and resistance to mechanical stress. Its discovery expanded understanding of how the cytoskeleton connects with membrane complexes in muscle biology.

Structurally, Syncoilin contains a central coiled-coil domain flanked by head and tail regions typical of intermediate filament proteins. This organization allows Syncoilin to form filamentous networks and link them to membrane-associated complexes. Syncoilin interacts with alpha-dystrobrevin, a component of the DAPC, and with Desmin, an intermediate filament protein in muscle. These interactions anchor the cytoskeleton to the sarcolemma, stabilizing muscle fibers during contraction and relaxation.

Functionally, Syncoilin contributes to muscle integrity and repair. It stabilizes the DAPC, facilitates force transmission, and supports the structural integrity of sarcomeres. Knockout mouse models show defects in muscle fiber organization, increased susceptibility to stress, and impaired regeneration. Syncoilin expression is upregulated during muscle injury and regeneration, suggesting a role in repair processes. Researchers use Syncoilin antibody to study cytoskeletal organization, muscle pathophysiology, and repair mechanisms.

Clinically, Syncoilin has been investigated in muscular dystrophies. Although mutations in SYNC are rare, misregulation of Syncoilin contributes to dystrophic phenotypes. Abnormal expression patterns are reported in Duchenne and Becker muscular dystrophies, linking Syncoilin to the pathogenesis of these conditions. Its presence at the neuromuscular junction also implicates it in synaptic stability. Syncoilin is a promising marker for muscle disease diagnostics and therapeutic monitoring.

Experimentally, Syncoilin antibody is used in western blotting to detect the ~64 kDa protein, in immunohistochemistry to visualize localization at the sarcolemma, and in immunofluorescence to assess cytoskeletal organization. Immunoprecipitation with Syncoilin antibody helps identify its binding partners, including DAPC components. NSJ Bioreagents supplies Syncoilin antibody as a robust reagent for research into cytoskeletal dynamics, muscular dystrophy, and muscle regeneration.

UniProt: Q9EPM5