TTLL5 antibody (AA 111-1015)

Catalog No. ABIN7870111

The Rabbit Polyclonal anti-TTLL5 antibody (ABIN7870111) specifically detects TTLL5 in ELISA and WB. The antibody is reactive with Human samples.

Quick Overview for TTLL5 antibody (AA 111-1015) (ABIN7870111)

Target: TTLL5 (Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTLL5 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-TTLL5 Antibody

Binding Specificity: AA 111-1015

Purpose: TTLL5 Antibody / Tubulin tyrosine ligase-like protein 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TTLL5 recombinant protein (Position: R111-E1015) was used as the immunogen for the TTLL5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TTLL5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TTLL5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TTLL5

Target: TTLL5 (Tubulin tyrosine Ligase-Like Family, Member 5 (TTLL5))

Alternative Name: TTLL5

Background: TTLL5 antibody detects Tubulin polyglutamylase TTLL5 (Tubulin tyrosine ligase-like protein 5), an enzyme that catalyzes post-translational glutamylation of alpha- and beta-tubulin, thereby regulating microtubule stability, motility, and interactions with associated proteins. Encoded by the TTLL5 gene on chromosome 14q24.3, this enzyme belongs to the TTLL family of tubulin-modifying enzymes responsible for adding glutamate side chains to tubulin and other cytoskeletal proteins. TTLL5 contains a tubulin-tyrosine ligase domain and a coiled-coil region that mediate enzymatic activity and substrate recognition. Through microtubule glutamylation, TTLL5 plays crucial roles in ciliary function, intracellular trafficking, and sensory perception.

TTLL5 is expressed in multiple tissues, including retina, testis, and brain, where it supports specialized microtubule structures such as photoreceptor connecting cilia and sperm flagella. In photoreceptor cells, TTLL5 regulates the glutamylation of ciliary axonemes and outer segment proteins critical for vision. Mutations in TTLL5 cause inherited retinal dystrophies, including cone-rod dystrophy and Leber congenital amaurosis, due to defective ciliary transport and photoreceptor degeneration. In spermatozoa, TTLL5 is essential for flagellar stability and motility, and its absence leads to asthenozoospermia and male infertility.

The TTLL5 antibody is widely used in cell biology, neurobiology, and reproductive research to study microtubule post-translational modification and ciliary biology. Western blot analysis identifies a 130 kilodalton band corresponding to the full-length enzyme, while immunofluorescence reveals localization along ciliary and cytoplasmic microtubules. Researchers employ this antibody to investigate TTLL5-dependent pathways controlling axonemal function and intracellular transport. Dysregulation of tubulin glutamylation has been implicated in neurodegenerative diseases and ciliopathies, making TTLL5 an important molecular marker for cytoskeletal regulation.

Functionally, TTLL5 acts with other TTLL family members to maintain the dynamic balance between glutamylation and deglutamylation, which modulates motor protein activity and vesicular transport. The TTLL5 antibody facilitates the study of these processes and enables precise mapping of TTLL5 expression across tissues. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reliable detection of TTLL5 in studies of ciliary structure, microtubule regulation, and human disease mechanisms.

UniProt: Q6EMB2