PET112L antibody (AA 113-443)

Catalog No. ABIN7870152

The Rabbit Polyclonal anti-PET112L antibody is suitable to detect PET112L in samples from Human and Mouse. It has been validated for WB, ELISA and FACS.

Quick Overview for PET112L antibody (AA 113-443) (ABIN7870152)

Target: PET112L (PET112) (PET112 Homolog (PET112))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PET112L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PET112L Antibody

Binding Specificity: AA 113-443

Purpose: GATB Antibody / Glutamyl-tRNA amidotransferase subunit B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GATB recombinant protein (Position: R113-E443) was used as the immunogen for the GATB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GATB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GATB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PET112L

Target: PET112L (PET112) (PET112 Homolog (PET112))

Alternative Name: GATB

Background: GATB antibody detects Glutamyl-tRNA amidotransferase subunit B, a mitochondrial enzyme subunit essential for tRNA-dependent amino acid amidation. Encoded by the GATB gene on chromosome 4q31.3, this protein forms part of the heterotrimeric GatCAB complex, which converts misacylated Glu-tRNA(Gln) and Asp-tRNA(Asn) into correctly charged Gln-tRNA(Gln) and Asn-tRNA(Asn), respectively. This transamidation reaction ensures translational accuracy and maintains proper protein synthesis in mitochondria and prokaryote-like systems.

Structurally, GATB is a 471-amino-acid mitochondrial protein of approximately 52 kilodaltons that contains an amidase domain and ATP-binding sites required for the transamidation process. It associates with the catalytic subunit GATC and the tRNA recognition subunit GATA to form the functional GatCAB complex. Within mitochondria, GATB localizes to the matrix, where it couples amino acid activation with tRNA modification to sustain accurate translation of mitochondrial-encoded proteins.

The GATB antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, protein biosynthesis fidelity, and mitochondrial genome maintenance. Western blot analysis detects a 52 kilodalton band corresponding to GATB, while immunofluorescence shows punctate mitochondrial staining that colocalizes with mitochondrial matrix markers such as HSP60. This antibody provides a dependable reagent for monitoring translational control and enzyme complex integrity in mitochondria.

Functionally, GATB ensures translational fidelity by catalyzing the amination of misacylated tRNAs, preventing incorporation of incorrect amino acids during protein synthesis. Loss or mutation of GATB disrupts mitochondrial translation and compromises respiratory chain assembly, leading to mitochondrial dysfunction, energy deficiency, and developmental disorders. Altered expression of GATB has been linked to neurodegenerative conditions and metabolic syndromes associated with impaired mitochondrial translation. The GATB antibody enables mechanistic studies of mitochondrial gene expression, enzyme complex assembly, and translational regulation under physiological and stress conditions. NSJ Bioreagents validates this antibody for its applications, ensuring reliable detection in mitochondrial and metabolic research applications.

UniProt: O75879