CEP55 antibody (AA 115-464)

Catalog No. ABIN7870211

This Rabbit Polyclonal antibody specifically detects CEP55 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for CEP55 antibody (AA 115-464) (ABIN7870211)

Target: CEP55 (Centrosomal Protein 55kDa (CEP55))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEP55 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CEP55 Antibody

Binding Specificity: AA 115-464

Purpose: CEP55 Antibody / Centrosomal protein of 55

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CEP55 recombinant protein (Position: E115-K464) was used as the immunogen for the CEP55 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CEP55 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CEP55 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CEP55

Target: CEP55 (Centrosomal Protein 55kDa (CEP55))

Alternative Name: CEP55

Background: CEP55 antibody recognizes Centrosomal protein of 55 kilodaltons, a key regulator of cytokinesis and abscission during cell division. CEP55 is essential for recruiting the ESCRT (endosomal sorting complex required for transport) machinery that mediates final membrane separation between daughter cells. The CEP55 antibody is widely used in cell cycle and cancer research to study centrosome function, mitotic exit, and cytokinetic regulation.

CEP55 is encoded by the CEP55 gene on human chromosome 10q23.33. The protein localizes to the centrosome and midbody during late mitosis, coordinating the final stages of cytokinesis. Structurally, CEP55 contains coiled-coil domains and a C-terminal ESCRT- and ALIX-binding region that enables its interaction with ESCRT-I component TSG101 and adaptor ALIX (PDCD6IP). These interactions recruit membrane scission complexes to the midbody, ensuring successful separation of daughter cells following chromosomal segregation.

The CEP55 antibody identifies a 55-60 kilodalton protein in western blot analysis, while immunofluorescence reveals strong midbody and centrosomal staining during telophase and cytokinesis. CEP55 overexpression is frequently observed in tumors, including breast, colon, and lung cancers, where it promotes uncontrolled proliferation and genomic instability. As a consequence, CEP55 is considered both a mitotic marker and a potential therapeutic target in oncology. In normal physiology, it functions as a checkpoint component ensuring that abscission only occurs after proper chromosomal segregation and spindle formation.

Functionally, CEP55 is phosphorylated by PLK1 and Aurora B kinases during mitosis, and these phosphorylation events regulate its recruitment timing to the midbody. Disruption of CEP55 impairs ESCRT recruitment, leading to cytokinesis failure and multinucleated cell formation. Beyond cytokinesis, CEP55 contributes to cilia disassembly and may play a role in signal transduction during cell cycle re-entry. NSJ Bioreagents offers a validated CEP55 antibody optimized for western blot and flow cytometry, providing researchers with a reliable reagent to study cell division, centrosome regulation, and cancer cell proliferation.

UniProt: Q53EZ4