ALOX5 antibody (pSer663) (HRP)

Catalog No. ABIN743552

The Rabbit Polyclonal anti-ALOX5 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect ALOX5 in samples from Human, Mouse and Rat.

Quick Overview for ALOX5 antibody (pSer663) (HRP) (ABIN743552)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ALOX5 Antibody (HRP)

Binding Specificity: pSer663

Specificity: This phosphorylation site is homologous to that of Ser664 in Mouse and Human.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: 5-Lipoxygenase

Background:

Synonyms: 5-LO, 5LPG, LOG5, 5-LOX, Arachidonate 5-lipoxygenase, 5-lipoxygenase, ALOX5

Background: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Gene ID: 240

UniProt: P09917