SECISBP2 antibody (AA 12-854)

Catalog No. ABIN7870304

This Rabbit Polyclonal antibody specifically detects SECISBP2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for SECISBP2 antibody (AA 12-854) (ABIN7870304)

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SECISBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SECISBP2 Antibody

Binding Specificity: AA 12-854

Purpose: SECISBP2 Antibody / Selenocysteine insertion sequence-binding protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SECISBP2 recombinant protein (Position: E12-L854) was used as the immunogen for the SECISBP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SECISBP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SECISBP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SECISBP2

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Alternative Name: SECISBP2

Background: SECISBP2 antibody detects Selenocysteine insertion sequence-binding protein 2, a cytoplasmic RNA-binding protein encoded by the SECISBP2 gene located on chromosome 9q22.2. SECISBP2 is essential for the synthesis of selenoproteins, a unique class of proteins that incorporate the amino acid selenocysteine at UGA codons during translation. The protein binds specifically to selenocysteine insertion sequence (SECIS) elements in the 3' untranslated regions (3' UTRs) of selenoprotein mRNAs, promoting selenocysteine incorporation by interacting with specialized translation factors. SECISBP2 is widely expressed, with high levels in liver, thyroid, and kidney where selenoprotein synthesis is most active.

Structurally, SECISBP2 contains RNA recognition motifs and a lysine-rich domain essential for binding SECIS elements. It forms part of a ribonucleoprotein complex with eukaryotic elongation factor EEFSEC and selenocysteine-specific tRNA (tRNA-Sec), ensuring accurate decoding of UGA as selenocysteine instead of a stop signal. SECISBP2 belongs to the SECIS-binding protein family, which includes SECISBP2L, a paralog with partially overlapping function. Co-localization studies show SECISBP2 distributed in cytoplasmic foci associated with ribosomes and endoplasmic reticulum-bound polysomes, indicating its role in co-translational selenoprotein synthesis.

Functionally, SECISBP2 regulates the expression of numerous selenoproteins involved in antioxidant defense, redox regulation, and thyroid hormone metabolism. These include glutathione peroxidases, thioredoxin reductases, and deiodinases. By controlling selenocysteine incorporation efficiency, SECISBP2 influences cellular responses to oxidative stress and selenium availability. During development, SECISBP2 expression supports organogenesis and metabolic maturation by maintaining proper selenoprotein production.

Mutations in SECISBP2 cause a multisystem disorder known as selenoprotein deficiency syndrome, characterized by impaired thyroid hormone activation, low plasma selenium levels, and muscle weakness. Reduced SECISBP2 function decreases synthesis of key antioxidant enzymes, leading to increased oxidative damage. Conversely, overexpression can enhance cellular resistance to oxidative stress by upregulating selenoprotein synthesis. Pathway associations include selenoamino acid metabolism, translational control, and oxidative stress response. Known binding partners include EEFSEC, SEPSECS, and tRNA-SEC, forming the machinery required for selenocysteine translation.

The SECISBP2 antibody from NSJ Bioreagents is an excellent reagent for studying selenium biology, redox regulation, and translational control of selenoproteins.

UniProt: Q96T21