ANKRD1 antibody (AA 120-319)

Catalog No. ABIN7870310

This Rabbit Polyclonal antibody specifically detects ANKRD1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ANKRD1 antibody (AA 120-319) (ABIN7870310)

Target: ANKRD1 (Ankyrin Repeat Domain 1 (Cardiac Muscle) (ANKRD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANKRD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ANKRD1 Antibody

Binding Specificity: AA 120-319

Purpose: ANKRD1 Antibody / Ankyrin repeat domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ANKRD1 recombinant protein (Position: D120-F319) was used as the immunogen for the ANKRD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ANKRD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ANKRD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ANKRD1

Target: ANKRD1 (Ankyrin Repeat Domain 1 (Cardiac Muscle) (ANKRD1))

Alternative Name: ANKRD1

Background: ANKRD1 antibody detects Ankyrin repeat domain-containing protein 1, encoded by the ANKRD1 gene. Ankyrin repeat domain-containing protein 1 is a transcriptional cofactor and stress-responsive protein that contributes to cardiac muscle biology, mechanical signaling, and gene regulation. ANKRD1 antibody provides researchers with a highly specific reagent for studying cardiomyocyte adaptation, hypertrophy, and muscle stress responses.

Ankyrin repeat domain-containing protein 1 belongs to the CARP (cardiac ankyrin repeat protein) family. Research using ANKRD1 antibody has shown that it is predominantly expressed in heart and skeletal muscle, where it localizes to the sarcomere, especially the I-band. Its ankyrin repeat domains allow it to interact with titin, myopalladin, and transcriptional regulators, linking mechanical stretch sensing to gene expression programs. This dual localization to sarcomeres and nuclei makes it an important signaling intermediary.

Studies with ANKRD1 antibody have revealed that its expression is induced by hypertrophic stress, hypoxia, and injury. In cardiomyocytes, ANKRD1 acts as both a structural stabilizer and a regulator of stress-induced transcriptional pathways. Elevated expression has been observed in dilated and hypertrophic cardiomyopathy, suggesting its potential role as a biomarker of heart disease.

Dysregulation of ANKRD1 has also been associated with muscular disorders and cancer. Research using ANKRD1 antibody has demonstrated that mutations in ANKRD1 contribute to congenital cardiomyopathy, while altered expression in tumors influences cell proliferation and invasion. Its pleiotropic functions in muscle and non-muscle tissues highlight its importance across biology.

ANKRD1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies expression in cardiac and skeletal tissue, immunohistochemistry highlights localization in diseased myocardium, and immunofluorescence demonstrates sarcomeric localization in cardiomyocytes. These methods make ANKRD1 antibody indispensable for cardiac biology research.

By supplying validated ANKRD1 antibody reagents, NSJ Bioreagents supports studies into sarcomere biology, transcriptional regulation, and disease. Detection of Ankyrin repeat domain-containing protein 1 provides researchers with insight into how cardiomyocytes adapt to stress and maintain function.

UniProt: Q15327

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Response to DNA Damage Stimulus