MMP20 antibody (AA 124-405)

Catalog No. ABIN7870366

The Rabbit Polyclonal anti-MMP20 antibody (ABIN7870366) specifically detects MMP20 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for MMP20 antibody (AA 124-405) (ABIN7870366)

Target: MMP20 (Matrix Metalloproteinase 20 (MMP20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MMP20 Antibody

Binding Specificity: AA 124-405

Purpose: MMP20 Antibody / Matrix metalloproteinase 20

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MMP20 recombinant protein (Position: Y124-K405) was used as the immunogen for the MMP20 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MMP20 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MMP20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MMP20

Target: MMP20 (Matrix Metalloproteinase 20 (MMP20))

Alternative Name: MMP20

Background: MMP20 antibody detects Matrix metalloproteinase-20, an extracellular endopeptidase that degrades enamel matrix proteins during tooth development. The UniProt recommended name is Matrix metalloproteinase-20 (MMP20). Also known as enamelysin, this enzyme belongs to the matrix metalloproteinase family responsible for remodeling extracellular matrix components during morphogenesis and tissue repair.

Functionally, MMP20 antibody identifies a 483-amino-acid secreted zinc-dependent protease synthesized as a zymogen that is activated by propeptide cleavage. MMP20 is specifically expressed in ameloblasts and odontoblasts, where it cleaves amelogenin, ameloblastin, and enamelin to enable proper enamel mineralization and organization.

The MMP20 gene is located on chromosome 11q22.2 and exhibits tissue-specific expression limited primarily to developing teeth. Its enzymatic activity contributes to normal enamel formation and maturation during odontogenesis.

Pathologically, mutations in MMP20 cause autosomal recessive amelogenesis imperfecta, a disorder characterized by defective enamel formation and tooth fragility. Altered MMP20 expression has also been implicated in oral cancer and tissue invasion. Research using MMP20 antibody supports studies in dental biology, extracellular matrix remodeling, and developmental biochemistry.

MMP20 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect matrix-degrading enzymes. NSJ Bioreagents provides MMP20 antibody reagents optimized for dental development, tissue remodeling, and protease regulation research.

Structurally, Matrix metalloproteinase-20 features a catalytic zinc-binding domain and a hemopexin-like domain responsible for substrate recognition. This antibody enables investigation of MMP20's function in enamel formation and extracellular matrix turnover.

UniProt: O60882