HTATSF1 antibody (AA 126-333)

Catalog No. ABIN7870394

This Rabbit Polyclonal antibody specifically detects HTATSF1 in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HTATSF1 antibody (AA 126-333) (ABIN7870394)

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HTATSF1 Antibody

Binding Specificity: AA 126-333

Purpose: HTATSF1 Antibody / HIV Tat-specific factor 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HTATSF1 recombinant protein (Position: H126-D333) was used as the immunogen for the HTATSF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HTATSF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HTATSF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1

Background: HTATSF1 antibody detects HIV Tat-specific factor 1, encoded by the HTATSF1 gene on chromosome 17q21.31. HTATSF1 antibody is commonly used in transcription biology, RNA processing, and viral research. HTATSF1 was originally identified as a cellular factor required for HIV-1 Tat-mediated transcriptional activation. It is now recognized as a general transcription elongation factor that associates with RNA polymerase II and influences RNA splicing, elongation, and processing.

Structurally, HTATSF1 is a nuclear protein containing RS-rich domains typical of splicing regulators, as well as multiple motifs for protein-protein and protein-RNA interactions. These domains allow HTATSF1 to associate with the transcription machinery and with spliceosomal components. Isoforms generated by alternative splicing may modulate transcriptional and splicing activities.

Functionally, HTATSF1 facilitates RNA polymerase II elongation by interacting with transcriptional elongation complexes and splicing factors. It coordinates co-transcriptional splicing, ensuring efficient coupling of RNA synthesis with processing. In the context of HIV infection, HTATSF1 enhances Tat-dependent transcriptional elongation, supporting viral replication. Researchers use HTATSF1 antibody to study transcription elongation, splicing regulation, and viral-host interactions.

Clinically, dysregulation of HTATSF1 has been implicated in cancer, where altered transcription elongation contributes to oncogenic gene expression. Because transcription elongation is critical for rapidly dividing cells, HTATSF1 is considered a potential therapeutic target. HTATSF1 also influences viral replication and could serve as a target for antiviral therapies. NSJ Bioreagents provides HTATSF1 antibody to support research in transcription biology, RNA processing, and infectious disease.

Experimentally, HTATSF1 antibody is used in western blotting to detect the protein, in immunohistochemistry to examine nuclear expression in tissues, and in chromatin immunoprecipitation to analyze transcriptional elongation complexes. Co-immunoprecipitation with HTATSF1 antibody identifies interaction partners in the elongation and splicing machinery.

UniProt: O43719