GANP antibody (AA 1275-1715)

Catalog No. ABIN7870405

The Rabbit Polyclonal anti-GANP antibody (ABIN7870405) specifically detects GANP in WB, ELISA, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GANP antibody (AA 1275-1715) (ABIN7870405)

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GANP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GANP Antibody

Binding Specificity: AA 1275-1715

Purpose: MCM3AP Antibody / Minichromosome maintenance complex-binding protein 3-associated protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GANP/MCM3AP recombinant protein (Position: R1275-H1715) was used as the immunogen for the MCM3AP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MCM3AP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MCM3AP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GANP

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Alternative Name: MCM3AP

Background: MCM3AP antibody detects Minichromosome maintenance complex-binding protein 3-associated protein, a nuclear factor involved in DNA replication regulation, chromatin modification, and mRNA export. The UniProt recommended name is Minichromosome maintenance complex-binding protein 3-associated protein (MCM3AP), also known as germinal-center associated nuclear protein (GANP). MCM3AP is an important component of the TREX-2 complex, linking transcription to mRNA processing and nuclear export while influencing genomic stability.

Functionally, MCM3AP antibody identifies a 200 kDa protein that interacts with the MCM3 subunit of the MCM helicase complex, which is essential for DNA replication initiation. MCM3AP acts as a replication checkpoint regulator, controlling helicase loading and preventing aberrant origin firing. Beyond replication, MCM3AP functions as a scaffold protein that anchors mRNA export factors to nuclear pore complexes through its association with ENY2 and PCID2. This dual involvement in DNA synthesis and mRNA trafficking underscores its central role in coordinating gene expression and genome maintenance.

The MCM3AP gene is located on chromosome 21q22.3 and encodes a large multidomain protein containing a histone acetyltransferase (HAT)-like region and a nuclear localization signal. The HAT-like domain acetylates replication factors, including MCM3, thereby modulating their chromatin association. MCM3AP also contributes to immunoglobulin gene hypermutation in germinal center B cells, reflecting its role in transcription-coupled mutagenesis. Its expression is elevated in proliferating lymphocytes, embryonic cells, and cancer tissues, where it supports rapid DNA replication and transcriptional activity.

In addition to its nuclear functions, MCM3AP has been implicated in mRNA surveillance and export. Through the TREX-2 complex, it connects RNA polymerase II transcription sites to nuclear pore channels, facilitating efficient export of mature mRNA to the cytoplasm. Loss of MCM3AP disrupts mRNA export, leading to nuclear accumulation of transcripts and impaired protein synthesis. Knockdown studies demonstrate that MCM3AP depletion results in DNA replication stress, S-phase arrest, and genomic instability.

MCM3AP antibody is widely used in research investigating DNA replication control, mRNA processing, and chromatin dynamics. Applications include western blotting, chromatin immunoprecipitation (ChIP), and confocal microscopy to analyze nuclear localization and replication factor interactions. In cancer studies, MCM3AP is explored as a potential biomarker of proliferation and genome integrity. It has been shown to enhance cell cycle progression and influence gene expression programs that support tumor growth and immune function.

Structurally, MCM3AP contains coiled-coil and zinc finger domains that mediate protein-protein interactions within the replication machinery and nuclear pore complexes. Post-translational modifications such as phosphorylation and acetylation regulate its activity during different cell cycle phases. NSJ Bioreagents provides MCM3AP antibody reagents validated for use in replication biology, epigenetics, and RNA export studies.

UniProt: O60318

Pathways: Protein targeting to Nucleus