MVK antibody (AA 13-394)

Catalog No. ABIN7870472

This Rabbit Polyclonal antibody specifically detects MVK in WB, ELISA, IHC, IF, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for MVK antibody (AA 13-394) (ABIN7870472)

Target: MVK (Mevalonate Kinase (MVK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MVK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MVK Antibody

Binding Specificity: AA 13-394

Purpose: MVK Antibody / Mevalonate kinase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MVK recombinant protein (Position: K13-D394) was used as the immunogen for the MVK antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MVK antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MVK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MVK

Target: MVK (Mevalonate Kinase (MVK))

Alternative Name: MVK

Background: MVK antibody detects Mevalonate kinase, a key enzyme in the mevalonate pathway responsible for cholesterol and isoprenoid biosynthesis. MVK catalyzes the phosphorylation of mevalonic acid to 5-phosphomevalonate, a crucial step in the production of sterols and nonsterol isoprenoids. The MVK antibody is widely used in metabolic, genetic, and biochemical research to study cholesterol regulation, inflammation, and metabolic disorders.

MVK is encoded by the MVK gene located on human chromosome 12q24.11. The protein is approximately 396 amino acids long and localizes to the peroxisome and cytoplasm. MVK activity is regulated by feedback inhibition through cholesterol and isoprenoid intermediates, ensuring metabolic balance in lipid biosynthesis. The enzyme participates in the early steps of the mevalonate pathway, linking carbohydrate metabolism to lipid production.

The MVK antibody detects a 43 kilodalton protein by western blot and reveals diffuse cytoplasmic and peroxisomal staining under immunofluorescence microscopy. MVK provides precursors for essential biomolecules such as coenzyme Q, dolichol, and prenylated proteins, which are required for membrane integrity, protein glycosylation, and signal transduction.

Mutations in MVK cause mevalonic aciduria and hyperimmunoglobulinemia D syndrome, characterized by recurrent fever and inflammation due to impaired isoprenoid synthesis and excessive accumulation of mevalonic acid. MVK deficiency disrupts protein prenylation, mitochondrial function, and immune regulation, leading to autoinflammatory symptoms. The enzyme's activity is thus essential for cellular metabolism, immune homeostasis, and energy balance.

Because of its central role in cholesterol metabolism and inflammation, MVK is a crucial target for understanding metabolic regulation and disease pathogenesis. NSJ Bioreagents provides a validated MVK antibody optimized for its applications, supporting research into lipid metabolism, genetic disorders, and therapeutic development.

UniProt: Q03426