TTDN1 antibody (AA 135-179)

Catalog No. ABIN7870539

The Rabbit Polyclonal anti-TTDN1 antibody is suitable to detect TTDN1 in samples from Human. It has been validated for WB, ELISA, ICC and IF.

Quick Overview for TTDN1 antibody (AA 135-179) (ABIN7870539)

Target: TTDN1 (MPLKIP) (M-Phase Specific PLK1 Interacting Protein (MPLKIP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTDN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TTDN1 Antibody

Binding Specificity: AA 135-179

Purpose: MPLKIP Antibody / M phase specific PLK1 interacting protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MPLKIP recombinant protein (Position: E135-C179) was used as the immunogen for the MPLKIP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MPLKIP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MPLKIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TTDN1

Target: TTDN1 (MPLKIP) (M-Phase Specific PLK1 Interacting Protein (MPLKIP))

Alternative Name: MPLKIP

Background: MPLKIP antibody is used to study M phase specific PLK1 interacting protein, a small nuclear protein that binds the mitotic kinase PLK1 and helps coordinate chromosome dynamics and cell cycle progression. By engaging Polo like kinase 1 during M phase, the protein contributes to proper spindle function and timely transition through mitosis, thereby preserving genome integrity. Beyond cell division, M phase specific PLK1 interacting protein participates in gene regulatory programs and has been linked to transcription-coupled processes that safeguard cell identity and stress adaptation.

The protein localizes to the nucleus and perinuclear regions, where it interfaces with chromatin and cell cycle machinery. Loss of function mutations in the MPLKIP gene, historically known as TTDN1, cause trichothiodystrophy non photosensitive type 1, a disorder characterized by brittle hair and developmental features, underscoring its role in nuclear homeostasis. Experimental systems suggest that M phase specific PLK1 interacting protein acts as a scaffold that positions kinases and effectors to ensure accurate phosphorylation timing, with downstream consequences for cohesion, condensation, and checkpoint signaling.

In the laboratory, researchers use MPLKIP antibody for immunoblotting to monitor expression across the cell cycle, for immunofluorescence to visualize nuclear patterns in interphase and mitosis, and for co-immunoprecipitation to examine complexes with PLK1 and associated regulators. When paired with synchronization, kinase inhibitors, or auxin-degron depletion, the antibody supports dissection of cause-and-effect relationships between protein abundance, phosphorylation events, and mitotic fidelity. In disease models, MPLKIP antibody enables evaluation of variant impact on stability and localization, clarifying genotype-phenotype mechanisms relevant to neurodevelopment and ectodermal biology.

The MPLKIP antibody from NSJ Bioreagents is optimized for consistent detection in common cell lines and primary cells, integrating with workflows that combine imaging, phospho-proteomics, and transcriptomics. By anchoring measurements of a PLK1-interacting scaffold, teams can connect mitotic control to transcriptional resets that follow cell division, and they can probe how nuclear organization influences tissue development and stress resilience.

UniProt: Q8TAP9