PAX8 antibody (AA 137-450)

Catalog No. ABIN7870578

The Rabbit Polyclonal anti-PAX8 antibody is suitable to detect PAX8 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for PAX8 antibody (AA 137-450) (ABIN7870578)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PAX8 Antibody

Binding Specificity: AA 137-450

Purpose: PAX8 Antibody / Paired box gene 8

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PAX8 recombinant protein (Position: Q137-L450) was used as the immunogen for the PAX8 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PAX8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PAX8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: PAX8 antibody detects Paired box protein Pax-8, encoded by the PAX8 gene. Paired box protein Pax-8 is a transcription factor that plays a critical role in thyroid gland development, renal morphogenesis, and Mullerian duct differentiation. PAX8 antibody provides researchers with a vital reagent for studying transcriptional regulation, organogenesis, and tumor diagnostics.

PAX8 belongs to the paired box (PAX) family of transcription factors, which share a conserved DNA-binding paired domain. Research using PAX8 antibody has shown that it is essential for thyroid follicular cell development and regulates transcription of thyroid-specific genes including thyroglobulin and thyroperoxidase. These functions are crucial for thyroid hormone biosynthesis and endocrine regulation.

Studies with PAX8 antibody have revealed that PAX8 is also expressed in kidney and Mullerian duct-derived tissues, where it regulates organ formation and differentiation. This broad developmental role emphasizes its importance in multiple systems. PAX8 knockout mice display thyroid agenesis and renal malformations, demonstrating its essential developmental functions.

Dysregulation of Paired box protein Pax-8 has been associated with thyroid disease, infertility, and cancer. Research using PAX8 antibody has shown that PAX8 mutations contribute to congenital hypothyroidism and thyroid dysgenesis. In oncology, PAX8 is frequently expressed in thyroid carcinomas, renal cell carcinomas, and ovarian carcinomas, making it a reliable immunohistochemical marker for tumor diagnosis.

PAX8 antibody is widely applied in immunohistochemistry, western blotting, and chromatin immunoprecipitation. Immunohistochemistry highlights diagnostic expression in thyroid and renal tumors, western blotting quantifies protein levels, and ChIP studies identify DNA binding sites. These applications make PAX8 antibody indispensable in developmental biology and cancer pathology.

By providing validated PAX8 antibody reagents, NSJ Bioreagents supports studies into thyroid development, transcriptional regulation, and oncology. Detection of Paired box protein Pax-8 provides researchers with insight into how transcription factors govern organogenesis and disease.

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation