PGK1 antibody (AA 138-337)

Catalog No. ABIN7870590

The Rabbit Polyclonal anti-PGK1 antibody is suitable to detect PGK1 in samples from Zebrafish (Danio rerio). It has been validated for WB and IHC (p).

Quick Overview for PGK1 antibody (AA 138-337) (ABIN7870590)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PGK1 Antibody

Binding Specificity: AA 138-337

Purpose: Zebrafish Pgk1 Antibody / Phosphoglycerate kinase 1

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Pgk1 recombinant protein (amino acids N138-N337) was used as the immunogen for the Zebrafish Pgk1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Pgk1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Pgk1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: Pgk1

Background: PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

UniProt: Q7ZV29

Pathways: Cellular Glucan Metabolic Process