NIP7 antibody (AA 14-180)

Catalog No. ABIN7870616

This Rabbit Polyclonal antibody specifically detects NIP7 in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for NIP7 antibody (AA 14-180) (ABIN7870616)

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NIP7 Antibody

Binding Specificity: AA 14-180

Purpose: NIP7 Antibody / Nucleolar pre-rRNA processing protein NIP7

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NIP7 recombinant protein (Position: E14-T180) was used as the immunogen for the NIP7 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NIP7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NIP7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NIP7

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Alternative Name: NIP7

Background: NIP7 antibody detects Nucleolar pre-rRNA processing protein NIP7, a highly conserved factor involved in the biogenesis of the small ribosomal subunit. NIP7 participates in pre-rRNA processing and ribosome assembly within the nucleolus, contributing to the accurate formation of mature 18S rRNA. The NIP7 antibody is commonly used in molecular and cellular biology research to examine ribosome biogenesis, nucleolar function, and RNA processing mechanisms that sustain cell growth and proliferation.

NIP7 is encoded by the NIP7 gene located on human chromosome 18q12.1. The protein is approximately 20 kilodaltons in size and localized predominantly in the nucleolus, where it associates with pre-ribosomal particles. Structurally, NIP7 contains an Sm-like fold and RNA-binding motifs enabling it to interact with ribosomal RNA precursors and small nucleolar RNAs. It forms complexes with NOP8 and RRP12, essential for the processing of 20S pre-rRNA and export of pre-40S subunits to the cytoplasm. These interactions make NIP7 a critical factor in maintaining ribosome synthesis efficiency.

The NIP7 antibody is valuable for detecting nucleolar proteins by western blot or immunocytochemistry. Western blot typically shows a distinct band at approximately 25 kilodaltons, while immunofluorescence highlights punctate nucleolar localization that co-stains with fibrillarin or nucleolin. Depletion of NIP7 disrupts 18S rRNA maturation, leading to defective ribosome assembly and impaired cell growth. Beyond ribosome synthesis, NIP7 may participate in broader RNA processing pathways, linking ribosome production to cell cycle progression and metabolic control.

NIP7 is evolutionarily conserved from yeast to humans, emphasizing its fundamental role in RNA biology. Altered expression has been noted in certain cancers and stress conditions that perturb ribosome biogenesis. NSJ Bioreagents offers a validated NIP7 antibody optimized for western blot and flow cytometry, enabling detailed exploration of ribosome formation, RNA maturation, and nucleolar organization in eukaryotic cells.

UniProt: Q9Y221

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly