SIRT6 antibody (AA 14-355)

Catalog No. ABIN7870627

The Rabbit Polyclonal anti-SIRT6 antibody (ABIN7870627) specifically detects SIRT6 in WB, ELISA, ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for SIRT6 antibody (AA 14-355) (ABIN7870627)

Target: SIRT6 (Sirtuin 6 (SIRT6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SIRT6 Antibody

Binding Specificity: AA 14-355

Purpose: SIRT6 Antibody / Sirtuin 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SIRT6 recombinant protein (Position: D14-S355) was used as the immunogen for the SIRT6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SIRT6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SIRT6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Alternative Name: SIRT6

Background: SIRT6 antibody detects Sirtuin 6, a nuclear NAD+-dependent deacetylase that regulates genomic stability, metabolism, and lifespan. The UniProt recommended name is NAD-dependent protein deacylase sirtuin 6 (SIRT6). This enzyme belongs to the sirtuin family of class III histone deacetylases, which use NAD+ as a cofactor to remove acyl groups from lysine residues on histone and non-histone proteins.

Functionally, SIRT6 antibody recognizes a chromatin-associated protein that deacetylates histone H3 at lysine 9 and lysine 56, maintaining telomere integrity and DNA repair efficiency. SIRT6 also promotes base excision repair and regulates transcription by modulating chromatin compaction. It interacts with PARP1 and SNF2H to coordinate double-strand break repair and suppress genomic instability. Beyond chromatin regulation, SIRT6 controls glucose and lipid metabolism by repressing glycolytic gene expression and enhancing mitochondrial respiration.

The SIRT6 gene, located on chromosome 19p13.3, encodes a 355-amino-acid enzyme localized predominantly in the nucleus. SIRT6 expression is enriched in metabolically active tissues such as heart, liver, and brain. It modulates aging-related pathways by influencing insulin sensitivity, inflammation, and DNA repair. Deficiency of SIRT6 in mice causes premature aging, metabolic dysregulation, and shortened lifespan.

Pathologically, dysregulated SIRT6 activity has been implicated in cancer, diabetes, cardiovascular disease, and neurodegeneration. In cancer, SIRT6 may act as a tumor suppressor by limiting glycolysis and maintaining genomic integrity. In metabolic disorders, altered SIRT6 function disrupts energy homeostasis and inflammatory signaling. Research using SIRT6 antibody supports studies of chromatin remodeling, aging, and metabolic control.

SIRT6 antibody is validated for use in its applications, and it is widely applied in DNA repair and transcriptional regulation studies. NSJ Bioreagents supplies SIRT6 antibody reagents optimized for accurate detection of nuclear sirtuin activity and longevity-related mechanisms.

Structurally, SIRT6 contains a conserved Rossmann-fold domain responsible for NAD+ binding and a zinc-binding module stabilizing its catalytic core. The enzyme exhibits both deacetylase and long-chain deacylase activity, extending its regulatory range to lipid-modified substrates. This antibody aids in investigating SIRT6i 1/2s central role in chromatin biology and metabolic regulation.

UniProt: Q8N6T7