SMAP2 antibody (AA 142-429)

Catalog No. ABIN7870691

This Rabbit Polyclonal antibody specifically detects SMAP2 in WB, ELISA, IP and FACS. It exhibits reactivity toward Human.

Quick Overview for SMAP2 antibody (AA 142-429) (ABIN7870691)

Target: SMAP2 (Small ArfGAP2 (SMAP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-SMAP2 Antibody

Binding Specificity: AA 142-429

Purpose: SMAP2 Antibody / Small ArfGAP 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SMAP2 recombinant protein (Position: K142-K429) was used as the immunogen for the SMAP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SMAP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SMAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SMAP2

Target: SMAP2 (Small ArfGAP2 (SMAP2))

Alternative Name: SMAP2

Background: SMAP2 antibody detects Small ArfGAP 2, an ADP-ribosylation factor GTPase-activating protein that regulates membrane trafficking and clathrin-mediated endocytosis. The UniProt recommended name is Small ArfGAP 2 (SMAP2). This cytosolic protein functions as a regulator of ARF family small GTPases, controlling vesicle formation and cargo sorting at the trans-Golgi network and plasma membrane.

Functionally, SMAP2 antibody identifies a 429-amino-acid protein containing an ArfGAP domain and a C-terminal clathrin-binding region. SMAP2 stimulates GTP hydrolysis on ARF1 and ARF6, facilitating vesicle uncoating and recycling. It plays a key role in synaptic vesicle turnover, receptor endocytosis, and intracellular trafficking, ensuring efficient communication between organelles.

The SMAP2 gene, located on chromosome 1p34.1, is widely expressed in brain, heart, and other tissues with active vesicular transport. In neurons, SMAP2 localizes to presynaptic terminals, where it interacts with clathrin and adaptor proteins to regulate synaptic vesicle cycling. Its activity coordinates endocytosis and exocytosis, maintaining synaptic plasticity and receptor availability.

Pathologically, aberrant SMAP2 expression or dysfunction may contribute to neurological disorders, including synaptic transmission defects and neurodevelopmental abnormalities. Its regulatory role in membrane dynamics also links it to cancer cell migration and secretion. Research with SMAP2 antibody provides insight into ARF signaling, vesicle recycling, and cellular communication mechanisms.

SMAP2 antibody is suitable for western blotting, immunocytochemistry, and immunoprecipitation to detect SMAP2 in cultured cells and tissues. NSJ Bioreagents offers validated SMAP2 antibody reagents designed for studies of ARF GTPase signaling, endocytosis, and neuronal function.

Structurally, SMAP2 contains an N-terminal ArfGAP catalytic domain that interacts with GTP-bound ARF, accelerating GTP hydrolysis, and a C-terminal region that binds clathrin and adaptor proteins. This modular structure allows SMAP2 to act as a molecular bridge between vesicle formation and signaling regulation. This antibody aids in understanding SMAP2i 1/2s contribution to intracellular trafficking and membrane remodeling.

UniProt: Q8WU79